SCHISTOSOMA-MANSONI - THE PRESENCE AND POTENTIAL USE OF OPIATE-LIKE SUBSTANCES
SCHISTOSOMA-MANSONI - THE PRESENCE AND POTENTIAL USE OF OPIATE-LIKE SUBSTANCES
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DOI：
10.1006/expr.1995.1110
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发表时间：
1995-09-01
影响因子：
2.1
通讯作者：
STEFANO, GB
中科院分区：
文献类型：
--
作者：
LEUNG, MK;DISSOUS, C;STEFANO, GB
本研究表明，经高效液相色谱分离和鉴定后，曼氏血吸虫体内存在类似吗啡和可待因的分子。一种合适的商业化抗体。此外，内源性物质，对应于吗啡，在诱导免疫细胞圆形和静止的能力方面模仿真正的吗啡，这一作用是纳洛酮敏感的。与吗啡类物质相比，在高浓度下没有发现可待因样物质，这表明，与哺乳动物和紫贻贝一样，可待因有可能迅速转化为吗啡。与人类白细胞共孵育会增加成虫体内这种物质的水平，这表明存在一个正反馈回路。最后，EDTA，一种二价阳离子的螯合剂，在蠕虫合成类似吗啡的物质方面有很强的刺激作用，这一点在它存在时这种物质的水平更高。综上所述，结果表明，这种寄生虫可能利用这种免疫下调分子来努力逃避宿主的免疫监视，以及抑制针对自己的免疫反应。(C)1995年学术出版社。
The present study demonstrates that morphine- and codeine-like molecules are present in Schistosoma mansoni following HPLC separation and identification with. an appropriate commercially available antibody. Furthermore, the endogenous material, corresponding to morphine, mimics authentic morphine in Its ability to induce immunocyte rounding and immobility, an action that is naloxone sensitive. The codeine-like material is not found at high concentrations compared to the morphine-like material, indicating, as in mammals and Mytilus edulis, the potential rapid conversion of codeine to morphine. Coincubation with human leukocytes increases the endogenous level of this material in adult worms, indicating the presence of a positive feedback loop. Last, EDTA, a chelator of divalent cations, has a strong stimulating effect in the synthesis of morphine-like material by the worm as noted by higher levels of this material in its presence. Taken together, the results suggest that this parasite may utilize this immune downregulating molecule in its effort to escape host immunosurveillance as well as in inhibiting an immune response directed against itself. (C) 1995 Academic Press, Inc.