DISTRIBUTION OF HEMAGGLUTININ AND NEURAMINIDASE ON INFLUENZA VIRIONS AS REVEALED BY IMMUNOELECTRON MICROSCOPY
DISTRIBUTION OF HEMAGGLUTININ AND NEURAMINIDASE ON INFLUENZA VIRIONS AS REVEALED BY IMMUNOELECTRON MICROSCOPY
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DOI：
10.1016/0042-6822(86)90084-x
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发表时间：
1986-02-01
期刊：
影响因子：
3.7
通讯作者：
WEBSTER, RG
中科院分区：
文献类型：
--
作者：
MURTI, KG;WEBSTER, RG
用流感病毒血凝素（HA）或神经氨酸酶（NA）特异性单克隆抗体进行免疫电镜研究，以确定病毒粒子上两个表面刺突的分布。间接免疫金染色显示HA均匀分布在病毒粒子上，NA分布在离散区域。交联和低温研究反对HA和NA在抗体附着后重新分布，并表明流感病毒上的NA以斑块形式发生。
Monoclonal antibodies specific for the hemagglutinin (HA) or the neuraminidase (NA) of influenza viruses were used in immunoelectron microscopic studies to determine the distribution of the two surface spikes on the virion. Indirect immunogold staining revealed that the HA is uniformly distributed on the virion while the NA occurs in discrete areas. Crosslinking and low temperature studies argue against redistribution of the HA and NA after antibody attachment and indicate that the NA on influenza virus occurs in patches.