Enhancement of Hyaluronic Acid Production by Batch Culture of Streptococcus zooepidemicus via the addition of n-Dodecane as an Oxygen Vector
Enhancement of Hyaluronic Acid Production by Batch Culture of Streptococcus zooepidemicus via the addition of n-Dodecane as an Oxygen Vector
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DOI：
10.4014/jmb.0807.440
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发表时间：
2009-06-01
影响因子：
2.8
通讯作者：
Sun, Jun
中科院分区：
文献类型：
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作者：
Liu Long;Yang, Haiquan;Sun, Jun
本研究旨在探讨添加氧载体正十二烷对动物流行链球菌分批培养透明质酸（HA）的影响。由于培养液的高粘度，8 ~ 16 h微生物HA的产生受到氧传递系数K(L)a的限制，加入正十二烷可以提高氧传递系数。当培养基中添加正十二烷至终浓度为5% （v/v）时，8-16 h的K(L)a平均值提高到36 +/- 2 h(-1)，是未添加正十二烷对照的3.6倍。添加5% （v/v）的正十二烷，随着K(L)a和溶解氧（DO）的增加，与对照相比，HA产量增加了30%。此外，用两种数学模型比较了正十二烷不存在和不存在时氧的传质。如本文所述，使用正十二烷作为氧载体，为优化其他需氧生物聚合物产品提供了一种有效的替代方案，其中K(L)a通常是限制因素。
This study aimed to examine the influence of adding an oxygen vector, n-dodecane, on hyaluronic acid (HA) production by batch culture of Streptococcus zooepidemicus. Owing to the high viscosity of culture broth, microbial HA production during 8-16 h was limited by the oxygen transfer coefficient K(L)a, which could be enhanced by adding n-dodecane. With the addition of n-dodecane to the culture medium to a final concentration of 5% (v/v), the average value of K(L)a during 8-16 h was increased to 36 +/- 2 h(-1), which was 3.6 times that of the control without n-dodecane addition. With the increased K(L)a and dissolved oxygen (DO) by adding 5% (v/v) of n-dodecane, a 30% increase of HA production was observed compared with the control. Furthermore, the comparison of the oxygen mass transfer in the absence and presence of n-dodecane was conducted with two proposed mathematical models. The use of n-dodecane as an oxygen vector, as described in this paper, provides an efficient alternative for the optimization of other aerobic biopolymer productions, where K(L)a is usually a limiting factor.