Expression patterns of sialylated epitope recognized by KL-6 monoclonal antibody in ocular surface epithelium of normals and dry eye patients
Expression patterns of sialylated epitope recognized by KL-6 monoclonal antibody in ocular surface epithelium of normals and dry eye patients
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DOI：
10.1167/iovs.03-0988
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发表时间：
2004-07-01
影响因子：
4.4
通讯作者：
Ohashi, Y
中科院分区：
文献类型：
--
作者：
Hayashi, Y;Kao, WWY;Ohashi, Y
目的。确定KL-6表位（糖部分）与MUC1的关联及其在人非干眼和干眼眼表的分布。方法。使用单克隆抗体 (mAb) KL-6 对人眼表面进行免疫组织化学和免疫电子显微镜检查，该抗体可识别 MUC1 的碳水化合物表位。通过印迹细胞学检查24名非干眼志愿者和43名干眼患者的角膜和结膜细胞中KL-6表位的表达模式。结果。在角膜、球结膜和角膜缘上皮中，mAb KL-6 与浅层细胞的顶端细胞膜以及浅层细胞和翼细胞的细胞间隙发生反应。在基底上皮细胞的基底质膜中未观察到 mAb KL-6 的免疫反应性。印迹细胞学结果表明，24只非干眼中的13只的角膜上皮被mAb KL-6弱染色，而43只干眼中的42只显示马赛克图案。在非干眼症中，24 个球结膜上皮中有 19 个以蜂窝状图案表达 KL-6 表位。在轻度（17/19）和中度（17/17）干眼结膜中，KL-6表位呈现镶嵌图案。然而，严重干眼症中KL-6表位的表达下降，七名患者中的三名显示出马赛克模式，七名患者中的四名显示出弱标记的少数细胞。结论。这些发现表明，在轻度和中度干眼症中，顶角膜和结膜细胞对 MUC1 的唾液酸化 KL-6 表位进行上调。这种上调可能部分减轻干眼症中杯状细胞粘蛋白功能障碍引起的后果。值得注意的是，严重干眼症结膜中的 KL-6 表位下调，这一现象的部分原因可能是结膜上皮细胞的功能障碍。
PURPOSE. To determine the association of the KL-6 epitope (sugar moiety) with MUC1 and its distribution on the ocular surface of human non-dry and dry eyes.METHODS. The human ocular surface was examined immunohistochemically and immunoelectron microscopically using monoclonal antibody (mAb) KL-6, which recognize a carbohydrate epitope of MUC1. The expression patterns of KL-6 epitope in corneal and conjunctival cells were examined by impression cytology from 24 non-dry eye volunteers and 43 dry-eye patients.RESULTS. In the cornea, bulbar conjunctiva, and limbus epithelium, mAb KL-6 reacted to the apical cell membrane of superficial cells and the intercellular space of superficial and wing cells. No immune reactivity of mAb KL-6 was observed in the basal plasma membrane of basal epithelial cells. Results of impression cytology indicated that the corneal epithelium of 13 of 24 non-dry eyes was weakly stained by mAb KL-6, whereas 42 of 43 dry eyes showed a mosaic pattern. In non dry eyes, 19 of 24 bulbar conjunctival epithelia expressed the KL-6 epitope in a honeycomb pattern. In mild (17/19) and moderate (17/17) dry eye conjunctiva, the KL-6 epitope showed a mosaic pattern. However, the expression of KL-6 epitope decreased in severe dry eyes, showing a mosaic pattern in three of seven patients and labeling a few cells weakly in four of seven patients.CONCLUSIONS. These findings suggest that there is an upregulation of the sialylated KL-6 epitope of MUC1 by apical corneal and conjunctival cells in mild and moderate dry eyes. This upregulation may in part alleviate the consequences caused by goblet cell mucin dysfunction in dry eyes. It is noteworthy that the KL-6 epitope is downregulated in the conjunctiva of severe dry eyes, a phenomenon that may be explained in part by the malfunction of conjunctival epithelial cells.