Micro-injection of recombinant lysyl oxidase blocks oncogenic p21-Ha-Ras and progesterone effects on Xenopus laevis oocyte maturation
Micro-injection of recombinant lysyl oxidase blocks oncogenic p21-Ha-Ras and progesterone effects on Xenopus laevis oocyte maturation
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DOI：
10.1016/s0014-5793(97)01420-8
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发表时间：
1997-12-08
期刊：
影响因子：
3.5
通讯作者：
Gusmano, R
中科院分区：
文献类型：
--
作者：
Di Donato, A;Lacal, JC;Gusmano, R
先前的证据表明赖氨酸氧化酶具有抗肿瘤作用，主要在ras转化的细胞中，本研究证明重组赖氨酸氧化酶实际上能够拮抗p21- ha - ras诱导的非洲爪蟾卵母细胞成熟。赖氨酸氧化酶在黄体酮依赖性成熟过程中也有效，表明其位于Ras的下游。由活化的“成熟促进因子”诱导的成熟，通常由孕酮触发，也被赖氨酸氧化酶抑制。最后，赖氨酸氧化酶在成熟触发时并没有消除p42（Erk2）磷酸化，这表明Erk2的下游被阻断。进一步的研究表明赖氨酸氧化酶的作用依赖于蛋白质合成，因此可能是由新合成的蛋白质介导的。(C) 1997年欧洲生化学会联合会。
Previous evidence suggested an anti-oncogenic role for lysyl oxidase, mainly in ras-transformed cells, Here we prove that recombinant lysyl oxidase is actually able to antagonize p21-Ha-Ras-induced Xenopus laevis oocyte maturation. Lysyl oxidase was also effective on progesterone-dependent maturation, indicating a block lying downstream of Ras. Maturation induced by activated 'maturation promoting factor', normally triggered by progesterone, was also inhibited by lysyl oxidase, Finally, lysyl oxidase did not abolish p42(Erk2) phosphorylation upon maturation triggering, suggesting a block downstream of Erk2, Further investigation showed that lysyl oxidase action depends on protein synthesis and is therefore probably mediated by a newly synthesized protein. (C) 1997 Federation of European Biochemical Societies.