Diurnal pattern of rat pancreatic acinar cell replication
Diurnal pattern of rat pancreatic acinar cell replication
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DOI：
10.1007/s004410050997
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发表时间：
1998-02-01
影响因子：
3.6
通讯作者：
Elsässer, HP
中科院分区：
文献类型：
--
作者：
Biederbick, A;Elsässer, HP
在大鼠中，完全分化的胰腺腺泡细胞可以在适当的条件下进入细胞周期。本研究的目的是分析昼夜模式的腺泡细胞增殖作为食物摄入量和胆囊收缩素（CCK）的释放的函数，因为肽激素CCK是大鼠胰腺腺泡细胞复制的主要生理调节剂。胰腺腺泡细胞复制定量使用的S期标记增殖细胞核抗原（PCNA）的抗体。此外，腺泡细胞在S期后注射溴脱氧尿苷（BrdU）和随后的免疫组化染色的BrdU阳性细胞核检测。在标准饮食条件下，以及在禁食和再喂食的各种时间表后和应用CCK受体拮抗剂L-364，718后，在白天分析大鼠胰腺。早上6点到下午6点之间，PCNA标记指数为4.4 ± 0.9%，而在晚上9点至凌晨3点之间，PCNA标记指数升高，并在午夜前后达到峰值11.4%（平均值：7.8 ± 2.5%）。BrdU阳性细胞在午夜前后也比上午9点增加了一倍。在禁食大鼠中，腺泡细胞增殖被完全抑制，这种抑制可以通过注射CCK类似物雨蛙肽来克服。此外，CCK拮抗剂L-364，718导致与禁食相同的结果。在这里，我们第一次表明，有一个昼夜模式的胰腺腺泡细胞增殖大鼠，这是依赖于食物的摄入量和介导的CCK。
Fully differentiated pancreatic acinar cells can enter the cell cycle under appropriate conditions in the rat. The aim of this study was to analyse the diurnal pattern of acinar cell proliferation as a function of food intake and the release of cholecystokinin (CCK), because the peptide hormone CCK is a major physiological regulator of rat pancreatic acinar cell replication. Pancreatic acinar cell replication was quantitated using an antibody against the S-phase marker proliferating cell nuclear antigen (PCNA). In addition, acinar cells in S-phase were detected after injecting bromodeoxyuridine (BrdU) and subsequent immunohistochemical staining of BrdU-positive nuclei. Rat pancreata were analysed during the day under standard diet conditions, as well as after various schedules of fasting and refeeding and after the application of the CCK receptor antagonist L-364,718. Between 6 a.m. and 6 p.m., the PCNA labeling index was 4.4+/-0.9%, while between 9 p.m. and 3 a.m. the PCNA labeling index was elevated and reached peak values of 11.4% (mean value: 7.8+/-2.5%) around midnight. BrdU-positive cells also doubled around midnight, compared to the 9:00 a.m. value. In fasted rats, acinar cell proliferation was completely suppressed and this suppression could be overcome by injection of the CCK analog cerulein. In addition, the CCK antagonist L-364,718 led to the same results as fasting. Here we show for the first time that there is a diurnal pattern of pancreatic acinar cell proliferation in rats, which is dependent on food intake and is mediated by CCK.