Paraventricular nucleus stimulation causes gastroduodenal mucosal necrosis in the rat.
Paraventricular nucleus stimulation causes gastroduodenal mucosal necrosis in the rat.
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DOI：
10.1152/ajpregu.1988.255.5.r861
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发表时间：
1988-11
期刊：
影响因子：
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通讯作者：
A. Ferguson;P. Marcus;J. Spencer;J. Wallace
中科院分区：
文献类型：
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作者：
A. Ferguson;P. Marcus;J. Spencer;J. Wallace
本文观察了电刺激下丘脑室旁核（PVN）对SD大鼠胃肠道溃疡形成的影响。在PVN中进行电刺激（200微安，60 Hz，100微秒脉冲宽度）1小时，之后固定胃和十二指肠的样品用于随后的损伤的组织学评估。这种损伤在0（正常）至3（严重）量表上盲法评分。刺激PVN导致胃（平均损伤评分为1.8 +/- 0.3）和十二指肠（1.7 +/- 0.4）坏死和出血。相比之下，在紧邻PVN的区域中的类似刺激被发现对胃（0.3 +/- 0.1）或十二指肠（0.6 +/- 0.2）没有显著影响。在第二系列研究中，双侧迷走神经切断术后刺激PVN导致损伤评分（胃：0.2 +/- 0.1;十二指肠：0.1 +/- 0.1），与PVN刺激迷走神经完整组相比显著降低（P <0.05），但与PVN外区域刺激组相比无显著差异（P> 0.1）。这些数据表明，PVN传出神经影响延髓迷走神经节前神经元在急性胃十二指肠溃疡的发生中发挥重要作用。
The effects of stimulation in the paraventricular nucleus (PVN) of the hypothalamus on the development of gastrointestinal ulceration have been examined in urethan-anesthetized male Sprague-Dawley rats. Electrical stimulation (200 microA, 60 Hz, 100-microseconds pulse width) in the PVN was performed for 1 h, after which samples of the stomach and duodenum were fixed for subsequent histological assessment of damage. Such damage was scored, blindly, on a 0 (normal) to 3 (severe) scale. Stimulation in the PVN resulted in necrosis and hemorrhage in both the stomach (mean damage score of 1.8 +/- 0.3) and duodenum (1.7 +/- 0.4). In contrast, similar stimulation in regions immediately adjacent to the PVN was found to be without significant effect on the stomach (0.3 +/- 0.1) or duodenum (0.6 +/- 0.2). In a second series of studies PVN stimulation following bilateral vagotomy resulted in damage scores (stomach: 0.2 +/- 0.1; duodenum: 0.1 +/- 0.1) that were significantly reduced compared with the PVN-stimulated vagal-intact group (P less than 0.05) but were not significantly different from the group stimulated in regions outside the PVN (P greater than 0.1). These data suggest an important role for PVN efferents influencing medullary vagal preganglionic neurons in the development of acute gastroduodenal ulceration.