Histopathological and cell enzyme studies of calcium phosphate cements
Histopathological and cell enzyme studies of calcium phosphate cements
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DOI：
10.4012/dmj.23.613
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发表时间：
2004-12-01
影响因子：
2.5
通讯作者：
Murai, S
中科院分区：
文献类型：
--
作者：
Sugawara, A;Fujikawa, K;Murai, S
最近开发出不含磷酸四钙的新型自凝磷酸钙骨水泥（N-CPC）。以磷酸盐溶液为胶结液，N-CPCs在10分钟内硬化，形成羟基磷灰石作为凝固产物。本研究的目的是评估 N-CPC 和传统 CPC 的生物相容性（研究 I）和细胞酶活性（研究 II）。测试了四种实验水泥：（1）无水磷酸二钙（DCPA）和氧化钙； (2) DCPA和氢氧化钙； (3)磷酸三钙、碳酸钙； (4) DCPA和磷酸四钙。水泥(1)-(3)的水泥液使用磷酸盐溶液，水泥(4)的水泥液使用水。烧结羟基磷灰石颗粒(5)用作对照。将测试材料植入大鼠皮下。术后4周处死动物并进行组织病理学观察。水泥（2）和（3）没有表现出炎症反应，并且仅被非常薄的纤维结缔组织包围。 N-CPCs的组织病理学反应几乎相同并且与(4)和(5)相似。此外，还检查了由骨水泥 (3) 和 (4) 的存在引起的碱性磷酸酶 (ALP-ase) 活性对源自狗牙槽骨的成骨细胞样细胞的影响，因为 ALP-ase 活性与新骨形成密切相关。这些结果表明（3）和（4）与皮下组织高度相容，并表明这些水泥可以增强现在的骨形成。
New types of self-setting calcium phosphate cement (N-CPC), which do not contain tetracalcium phosphate, were recently developed. N-CPCs harden in 10 minutes with phosphate solution as the cement liquid, and form hydroxyapatite as the set product. The objectives of the present study were to evaluate the biocompatibility (Study I) and cell enzyme activity of N-CPCs and a conventional CPC (Study II). Four experimental cements were tested: (1) dicalcium phosphate anhydrous (DCPA) and calcium oxide; (2) DCPA and calcium hydroxide; (3) tricalcium phosphate and calcium carbonate; and (4) DCPA and tetracalcium phosphate. Phosphate solution was used as the cement liquid for cements (1)-(3), and water for cement (4). Sintered hydroxyapatite particles (5) were used as a control. The test materials were implanted subcutaneously in rats. Four weeks after operation, the animals were sacrificed and histopathological observations were performed. Cements (2) and (3) showed no inflammatory reaction, and were surrounded only by very thin fibrous connective tissues. The histopathological reactions of N-CPCs were nearly identical and were similar to (4) and (5). In addition, effects of alkaline phosphatase (ALP-ase) activity - invoked by the presence of cements (3) and (4) - on osteoblast-like cells derived from dog alveolar bone were also examined because ALP-ase activity is closely related to new bone formation. These results indicated that (3) and (4) were highly compatible with subcutaneous tissues and suggested that these cements may enhance now bone formation.