Pap Smear miR-92a-5p and miR-155-5p as potential diagnostic biomarkers of squamous intraepithelial cervical cancer.
Pap Smear miR-92a-5p and miR-155-5p as potential diagnostic biomarkers of squamous intraepithelial cervical cancer.
复制标题
DOI：
10.31557/apjcp.2021.22.4.1271
复制
发表时间：
2021-04-01
期刊：
影响因子：
--
通讯作者：
Zamani A
中科院分区：
文献类型：
--
作者：
Azimi T;Paryan M;Mondanizadeh M;Sarmadian H;Zamani A
宫颈癌是女性特有的发病率和死亡率都很高的疾病之一。宫颈癌的主要原因是感染了人乳头瘤病毒（HPV）。低度鳞状上皮内病变（LSIL）和高度鳞状上皮内病变（HSIL）通常由HPV感染引起。考虑到microRNA（miRNAs）作为多种癌症诊断生物标志物的作用，本研究的目的是确定LSIL和HSIL Pap样本中miR-92 a-5 p和miR-155- 5 p的表达水平。 经过初步的生物信息学研究，共75个样本（25个LSIL患者样本，25个HSIL患者样本和25个健康个体）进行RNA提取和cDNA合成。通过真实的时间PCR分析来评估LSIL、HSIL和健康个体的样品中证实的miRNA的表达水平。为了证明预测的miRNA作为新的生物标志物在LSIL和HSIL诊断中的作用，进行ROC曲线分析。 生物信息学结果显示miR-92 a-5 p和miR-155- 5 p靶向HPV E6和E7基因。LSIL患者宫颈涂片中miRNAs的表达水平显著高于健康对照组（35.36，P = 0.001）（62.23，P = 0.001）。相似的是，miR-92 a-5 p和miR-155- 5 p在HSIL患者中的表达水平显著高于健康人（33.62，P= 0.001）（69.07，P= 0.001）。虽然，miR-92 a-5 p水平（0.95，P = 0. 85)miR-155- 5 p（1.11，P = 0.84）在LSIL和HSIL患者中的表达无统计学差异。ROC曲线分析证实miR-92 a-5 p和miR-155- 5 p具有特异性和敏感性，可作为宫颈癌早期检测的新生物标志物。 这些数据表明，miR-92 a-5 p和miR-155- 5 p在LSIL和HSIL中上调，可以被认为是宫颈癌患者预后的预测生物标志物。
one of the female-specific diseases with a high incidence and mortality is cervical cancer. The main cause of cervical cancer is infection with Human papilloma virus (HPV). Low-grade squamous intraepithelial lesions (LSIL) and High-grade squamous intraepithelial lesions (HSIL) usually is caused by an HPV infection. Considering the role of microRNAs (miRNAs) as diagnostic biomarkers for a variety of cancers, the aim of this study was to determine miR-92a-5p and miR-155-5p expression levels in LSIL and HSIL Pap Smear samples. After initial bioinformatic studies, A total of 75 samples (25 samples of patients with LSIL, 25 patients with HSIL and 25 healthy individuals) were subjected to RNA extraction and cDNA synthesis. The expressions levels of confirmed miRNAs in samples of patients with LSIL, HSIL and healthy individuals were evaluated by Real time PCR analysis. To demonstration the role of predicted miRNAs as novel biomarkers in diagnosis of LSIL and HSIL, ROC curve analysis was done. Bioinformatics results showed that miR-92a-5p and miR-155-5p target the HPV E6 and E7 genes. The expression levels of these miRNAs were strikingly higher in Pap smear of patients with LSIL than in the healthy individuals (35.36, P = 0.001) (62.23, P = 0.001). Similarity, expression levels of miR-92a-5p and miR-155-5p were amazingly higher in patients with HSIL than in the healthy individuals (33.62, P= 0.001) (69.07, P= 0.001). Although, the levels of miR-92a-5p (0.95, P = 0. 85) and miR-155-5p (1.11, P = 0.84) exhibited no statistical differences between patients with LSIL and HSIL. Also, ROC curve analyses verified that miR-92a-5p and miR-155-5p are specific and sensitive and may serve as new biomarkers for the early detection of cervical cancer. These data suggest miR-92a-5p and miR-155-5p, which are upregulated in LSIL and HSIL, can be consider as predictive biomarkers for the prognosis of cervical cancer patients.