Beta adrenergic receptor stimulated prostacyclin synthesis in rabbit coronary endothelial cells is mediated by selective activation of phospholipase D: inhibition by adenosine 3'5'-cyclic monophosphate.
Beta adrenergic receptor stimulated prostacyclin synthesis in rabbit coronary endothelial cells is mediated by selective activation of phospholipase D: inhibition by adenosine 3'5'-cyclic monophosphate.
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兔冠状动脉内皮细胞中 β 肾上腺素能受体刺激的前列环素合成是通过选择性激活磷脂酶 D 介导的：腺苷 35-环单磷酸的抑制。
DOI：
--
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发表时间：
1997
影响因子：
3.5
通讯作者：
K. Malik
中科院分区：
文献类型：
--
作者：
Y. Ruan;H. Kan;K. Malik
Activation of beta adrenergic receptors in the isolated rabbit heart by catecholamines stimulates prostacyclin (PGI2) synthesis, which is inhibited by adenosine 3'5'-cyclic monophosphate (cAMP). The purpose of this study was to determine if activation of beta adrenergic receptors in cultured coronary endothelial cells (CEC) of rabbit heart with isoproterenol (ISOP) stimulates PGI2 synthesis and if cAMP inhibits the synthesis of this prostanoid and to investigate the underlying mechanism. Incubation of CEC with ISOP increased production of cAMP and PGI2, measured as immunoreactive cAMP and 6-keto-prostaglandin F1alpha, (6-keto-PGF1alpha), respectively. Forskolin, an activator of adenylyl cyclase, increased cAMP accumulation and inhibited ISOP-stimulated 6-keto-PGF1alpha synthesis. 8-(4-chlorophenyl-thio) cAMP also inhibited ISOP-induced 6-keto-PGF1alpha production. However, miconazole, an inhibitor of adenylyl cyclase, reduced cAMP accumulation and enhanced ISOP-stimulated 6-keto-PGF1alpha synthesis in CEC. ISOP-induced 6-keto-PGF1alpha synthesis was attenuated by C2-ceramide, an inhibitor of phospholipase D (PLD) by propranolol, a beta-AR antagonist that also inhibits phosphatidate phosphohydrolase and by the diacylglycerol lipase inhibitor 1,6-bis-(cyclohexyloximinocarbonylamino)-hexane (RHC 80267). Acetylcholine (ACh) induced 6-keto-PGF1alpha synthesis was also inhibited by these agents. Both ISOP and ACh increased PLD activity, which was inhibited by C2-ceramide but not by RHC 80267 or propranolol. ACh but not ISOP increased phospholipase A2 activity in CEC. ISOP- but not ACh-induced increase in PLD activity was attenuated by forskolin and 8-(4-chlorophenyl-thio)-adenosine 3'-5'-cyclic monophosphate and augmented by miconazole. These data suggest that beta adrenergic receptors activation promotes PGI2 synthesis in the CEC by selective activation of PLD and that cAMP decreases PGI2 synthesis by decreasing PLD activity. Moreover, beta adrenergic receptors activated PLD appears to be distinct from that stimulated by ACh.
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DOI：
10.1016/s0021-9258(18)54023-0
发表时间：
1993-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
V. Natarajan;M. Taher;B. Roehm;N. Parinandi;H. Schmid;Z. Kiss;J. Garcia
通讯作者：
V. Natarajan;M. Taher;B. Roehm;N. Parinandi;H. Schmid;Z. Kiss;J. Garcia
大鼠主动脉中内皮素 1 激活多种机制，包括磷脂酶 D。
DOI：
10.1152/ajpcell.1992.262.4.c941
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
--
作者：
Liu,Y;Geisbuhler,B;Jones,AW
通讯作者：
Jones,AW
DOI：
10.1073/pnas.79.2.495
发表时间：
1982
影响因子：
11.1
作者：
A. Brotherton;J. Hoak
通讯作者：
A. Brotherton;J. Hoak
cAMP 对异丙肾上腺素诱导的兔心脏前列环素合成的抑制调节。
DOI：
10.1152/ajpregu.1989.257.4.r771
发表时间：
1989
期刊：
The American journal of physiology
影响因子：
--
作者：
WilliamsJr,JL;Malik,KU
通讯作者：
Malik,KU
培养的主动脉内皮细胞中的花生四烯酸代谢。
影响因子：
5.8
作者：
Whorton,AR;Collawn,JB;Montgomery,ME;Young,SL;Kent,RS
通讯作者：
Kent,RS