5 alpha-reductase activity in cultured human dermal papilla cells from beard compared with reticular dermal fibroblasts.
5 alpha-reductase activity in cultured human dermal papilla cells from beard compared with reticular dermal fibroblasts.
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与网状真皮成纤维细胞相比，培养的胡须人真皮乳头细胞中的 5α-还原酶活性。
DOI：
10.1111/1523-1747.ep12874003
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Susumu Takayasu
中科院分区：
文献类型：
--
作者：
Satoshi Itami;S. Kurata;Susumu Takayasu
为阐明雄激素促进男性胡须生长的作用机制，测定了培养的人胡须毛乳头细胞和网状真皮成纤维细胞中5α-还原酶的活性。单层与50 NM的[1，2-~3H]-睾酮孵育。从培养基中提取类固醇，用薄层层析法进行分析。胡须毛乳头细胞中的主要代谢物是双氢睾酮(DHT)，它是雄激素靶组织中最有效的雄激素。相反，在网状真皮成纤维细胞中，DHT的生成量与雄烯二酮相似。胡须真皮乳头细胞的5α-还原酶活性是同一皮肤标本网状真皮成纤维细胞的3~5倍。胡须毛乳头细胞中5-α-还原酶的表观米氏常数为1.0×10~(-6)M，与典型的雄激素靶细胞--生殖器皮肤成纤维细胞的米氏常数相当。网状真皮成纤维细胞为4.0×10~(-5)M。而枕部头皮毛囊的毛乳头细胞中5α-还原酶的活性与同一皮肤样本的网状真皮成纤维细胞相似。这些结果有力地表明，胡须毛乳头细胞是雄激素靶细胞，DHT在男性胡须的生长中起着作用。
The activity of 5 alpha-reductase was assessed in cultured human beard dermal papilla cells and reticular dermal fibroblasts to elucidate the mechanism of androgen action in promoting the growth of beards in men. The monolayer was incubated with 50 nM of [1,2-3H]-testosterone. Steroids were extracted from the medium and analyzed by thin layer chromatography. The major metabolite in the beard dermal papilla cells was dihydrotestosterone (DHT), the most potent androgen in the androgen target tissue. By contrast, the amount of DHT formed was similar to that of androstenedione in reticular dermal fibroblasts. The 5 alpha-reductase activity in beard dermal papilla cells was three to five times as high as that in the reticular dermal fibroblasts from the same skin sample. The apparant Michaelis constant of 5 alpha-reductase in the beard dermal papilla cells was 1.0 X 10(-6) M, which was virtually equivalent to that of genital skin fibroblasts, typical androgen target cells. It was 4.0 X 10(-5) M in reticular dermal fibroblasts. By contrast, the activities of 5 alpha-reductase in dermal papilla cells from occipital scalp hair follicles were similar to those of reticular dermal fibroblasts of the same skin samples. These results strongly suggest that the beard dermal papilla cell is an androgen target cell, and that DHT plays a role in the growth of beards in men.