EXPRESSION OF THE GENES FOR THE FERRITIN-H AND FERRITIN-L SUBUNITS IN RAT-LIVER AND HEART - EVIDENCE FOR TISSUE-SPECIFIC REGULATIONS AT PRETRANSLATIONAL AND POSTTRANSLATIONAL LEVELS
EXPRESSION OF THE GENES FOR THE FERRITIN-H AND FERRITIN-L SUBUNITS IN RAT-LIVER AND HEART - EVIDENCE FOR TISSUE-SPECIFIC REGULATIONS AT PRETRANSLATIONAL AND POSTTRANSLATIONAL LEVELS
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DOI：
10.1042/bj2750813
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发表时间：
1991-05-01
影响因子：
4.1
通讯作者：
SCHIAFFONATI, L
中科院分区：
文献类型：
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作者：
CAIRO, G;RAPPOCCIOLO, E;SCHIAFFONATI, L
铁蛋白多聚体外壳中存在的铁蛋白轻链和重链亚基(L和H)的比例因组织不同而不同。为了确定肝脏中L的含量高于心脏中异铁蛋白的调控机制，我们从核糖核酸和蛋白质水平分析了大鼠这两种组织中铁蛋白基因的表达。在心脏中，在合成和积累水平上，L和H的数量之比约为1，与它们各自的mRNAs之间的比例相同。相比之下，在肝脏中，L-mRNAs和H-mRNAs之间的比例大约是。2，不能完全解释L在该组织中异铁蛋白的巨大优势。由于在肝脏中，L基因既不优先与多聚核糖体结合，也不比H-核糖体更有效地翻译，所以肝脏特异的异铁蛋白图谱似乎是由翻译前和翻译后机制共同决定的，而在心脏中，翻译后调节似乎并不相关，组织特异性模式取决于mRNA的积累水平。
The proportion of ferritin light-chain and heavy-chain subunits (L and H) present in the ferritin multimeric shell varies between different tissues. To identify the regulatory mechanisms responsible for the greater amount of L in liver than in heart isoferritins, we analysed ferritin-gene expression at the RNA and protein levels in these two tissues of the rat. In the heart the ratio between the amount of L and H, at the level both of synthesis and accumulation, is about 1 and is the same as the ratio between their respective mRNAs. In contrast, in the liver, the ratio between the L- and H-mRNAs is approx. 2 and cannot entirely explain the large predominance of L in isoferritins in this tissue. Since in the liver the L-mRNA is neither preferentially associated with polyribosomes nor translated more efficiently than its H- counterpart, it seems that the liver-specific isoferritin profile is determined by a combination of pre- and post-translational mechanisms, whereas in heart the post-translational regulation does not seem to be relevant and the tissue-specific pattern is determined at the level of mRNA accumulation.