Rice Homeodomain Protein WOX11 Recruits a Histone Acetyltransferase Complex to Establish Programs of Cell Proliferation of Crown Root Meristem[OPEN]
Rice Homeodomain Protein WOX11 Recruits a Histone Acetyltransferase Complex to Establish Programs of Cell Proliferation of Crown Root Meristem[OPEN]
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DOI：
10.1105/tpc.16.00908
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发表时间：
2017-05
期刊：
影响因子：
11.6
通讯作者：
Shaoli Zhou;Wei Jiang;F. Long;Saifeng Cheng;Wenjing Yang;Yu Zhao;Daoxiu Zhou
中科院分区：
文献类型：
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作者：
Shaoli Zhou;Wei Jiang;F. Long;Saifeng Cheng;Wenjing Yang;Yu Zhao;Daoxiu Zhou
水稻WOX11通过与组蛋白乙酰化复合体相互作用诱导允许染色质基因表达，从而促进冠根发育。茎生冠根是谷类植物的主要根系。以往的研究表明，乌斯切尔相关同源盒基因WOX11是促进水稻（Oryza sativa）冠根出芽和伸长的必要和充分条件。在这里，我们发现WOX11招募ADA2-GCN5组蛋白乙酰转移酶模块来激活冠根分生组织中的下游靶基因。水稻ADA2和GCN5基因在根分生组织中高度表达，对细胞分裂和生长至关重要。WOX11和ADA2-GCN5通常靶向并调控一组参与能量代谢、细胞壁生物合成和激素反应的根特异性基因，其中一些基因已知对根发育很重要。结果表明，WOX11募集ADA2-GCN5建立了冠根分生组织细胞分裂的基因表达程序，提示WOX11促进根分生组织发育的策略是允许性染色质修饰，包括组蛋白乙酰化。
Rice WOX11 promotes crown root development by interacting with a histone acetylation complex to induce permissive chromatin of gene expression. Shoot-borne crown roots are the major root system in cereals. Previous work has shown that the Wuschel-related homeobox gene WOX11 is necessary and sufficient to promote rice (Oryza sativa) crown root emergence and elongation. Here, we show that WOX11 recruits the ADA2-GCN5 histone acetyltransferase module to activate downstream target genes in crown root meristem. Rice ADA2 and GCN5 genes are highly expressed in root meristem and are shown to be essential for cell division and growth. WOX11 and ADA2-GCN5 commonly target and regulate a set of root-specific genes involved in energy metabolism, cell wall biosynthesis, and hormone response, some of which are known to be important for root development. The results indicate that the recruitment of ADA2-GCN5 by WOX11 establishes gene expression programs of crown root meristem cell division and suggest that permissive chromatin modification involving histone acetylation is a strategy for WOX11 to stimulate root meristem development.