PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST SEROTYPE STRAINS OF PSEUDOMONAS-AERUGINOSA
PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST SEROTYPE STRAINS OF PSEUDOMONAS-AERUGINOSA
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DOI：
10.1128/iai.55.5.1051-1057.1987
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发表时间：
1987-05-01
影响因子：
3.1
通讯作者：
SOUTHAM, GG
中科院分区：
文献类型：
--
作者：
LAM, JS;MACDONALD, LA;SOUTHAM, GG
产生了针对17种IATS血清型铜绿假单胞菌菌株中的12种的单克隆抗体。87杂交瘤克隆被分离，并分泌的抗体被发现是反应与福尔马林固定的全细胞和纯化的脂多糖的同源菌株在酶联免疫吸附测定。在这些单克隆抗体中，主要的抗体类别是免疫球蛋白M（IgM）（76%），尽管也产生了IgG2a和IgG3同种型的抗体。根据单克隆抗体对同源株全细胞的凝集能力，可将其进一步分为两类。发现凝集性单克隆抗体与同源脂多糖的O侧链进行免疫印迹，而发现非凝集性单克隆抗体与外膜蛋白相关脂多糖反应。单克隆抗体的血清分型的适用性进行了检查，并发现几种抗体凝集全细胞和免疫印迹与相应的血清型的囊性纤维化患者的临床分离株的O抗原。总之，已经产生了一组针对铜绿假单胞菌IATS血清型菌株的单克隆抗体。这些抗体代表了一个无价的免疫试剂库，其可应用于血清分型、表位作图、脂多糖结构测定和针对铜绿假单胞菌的保护研究。
Monoclonal antibodies against 12 of the 17 IATS serotype strains of Pseudomonas aeruginosa were produced. Eighty-seven hybridoma clones were isolated, and the antibodies secreted were found to be reactive with both Formalin-fixed whole cells and purified lipopolysaccharide of homologous strains in enzyme-linked immunosorbent assays. Among these monoclonal antibodies, the predominant antibody class was immunoglobulin M (IgM) (76%), although antibodies of the IgG2a and IgG3 isotypes were also produced. The monoclonal antibodies could further be divided into two groups based on their ability to agglutinate whole cells of homologous strains. The agglutinating monoclonal antibodies were found to immunoblot with the O side chains of homologous lipopolysaccharide, while the nonagglutinating monoclonal antibodies were found to be reactive with outer membrane protein-associated lipopolysaccharide. The applicability of monoclonal antibodies for serotyping was examined, and several antibodies were found to agglutinate whole cells and immunoblot with the O antigen of corresponding serotypes of clinical isolates from cystic fibrosis patients. In conclusion, a set of monoclonal antibodies against the IATS serotype strains of P. aeruginosa have been produced. These antibodies represent a bank of invaluable immunological reagents which may have application in serotyping, epitope mapping, lipopolysaccharide structural determination, and studies of protection against P. aeruginosa.