Effect of a substance P antagonist on capsaicin-induced nociceptive reflex in the isolated spinal cord-tail preparation of the rat.
Effect of a substance P antagonist on capsaicin-induced nociceptive reflex in the isolated spinal cord-tail preparation of the rat.
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P物质拮抗剂对大鼠离体脊髓尾制剂中辣椒素诱导的伤害性反射的影响。
DOI：
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发表时间：
1987
影响因子：
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通讯作者：
M. Yanagisawa
中科院分区：
文献类型：
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作者：
M. Otsuka;M. Yanagisawa
开发了新生大鼠的分离脊髓尾制剂并用于研究各种药物的作用。脐带和尾部分别灌注含氧人工脑脊液。在尾部施用少量辣椒素诱导腰腹侧根（L3-L5）的去极化反应持续约30秒。预先用含有前列腺素 E1 或 E2 的培养基灌注尾部，可增强辣椒素的刺激作用。通过在脊髓上施用吗啡、甲硫氨酸脑啡肽、强啡肽 (1-13)、生长抑素、腺苷、GABA 和 P 物质 (SP) 拮抗剂 [D-Arg1、D-Pro2、D-Trp7,9、Leu11]SP 可抑制辣椒素诱导的伤害性反射，并通过荷包牡丹碱增强。本制剂将有助于今后疼痛和镇痛药物的研究。
An isolated spinal cord-tail preparation of the newborn rat was developed and used for studying the effects of various drugs. The cord and the tail were separately perfused with oxygenated artificial cerebrospinal fluid. Application of capsaicin in a small amount to the tail induced a depolarizing response of the lumbar ventral root (L3-L5) lasting for about 30 sec. The stimulating action of capsaicin was potentiated by previous perfusion of the tail with a medium containing prostaglandin E1 or E2. The capsaicin-induced nociceptive reflex was depressed by application to the spinal cord of morphine, Met-enkephalin, dynorphin (1-13), somatostatin, adenosine, GABA and a substance P (SP) antagonist [D-Arg1, D-Pro2, D-Trp7,9, Leu11]SP, and potentiated by bicuculline. The present preparation will be useful for the future studies on pain and analgesic drugs.