Potential HIV-1 target cells in the human penis
Potential HIV-1 target cells in the human penis
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DOI：
10.1097/01.aids.0000237364.11123.98
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发表时间：
2006-07-13
期刊：
影响因子：
3.8
通讯作者：
Short, Roger V.
中科院分区：
文献类型：
--
作者：
McCoombe, Scott G.;Short, Roger V.
目的：研究人类阴茎中HIV-1受体的分布和角质化程度。设计：福尔马林固定的阴茎，从9个未包皮环切的尸体。包皮取自21名因社会原因接受择期包皮环切术的健康成年男性。在8名男性死亡后24小时内获得了未割包皮的阴茎。所有组织进行角蛋白和HIV-1 receptors.Methods染色：阴茎从9福尔马林固定的尸体，年龄64-80岁，来自解剖学系，墨尔本大学。包皮取自21名年龄在18-64岁的男性，他们在澳大利亚墨尔本的共济会或慈悲私立医院进行了包皮环切术。从墨尔本的维多利亚法医学院获得8名年龄在23-63岁的未行包皮环切术男性的新鲜阴茎尸检标本。结果：HIV-1受体阳性细胞分布于所有阴茎上皮细胞，但郎格罕细胞以内包皮和系带最浅。内包皮角蛋白层（1.8 ± 0.1单位）明显薄于外包皮（3.3 ± 0.1单位）和龟头（3.3 ± 0.2单位），P <0.05。结论：包皮和系带内表面的表皮郎格罕细胞受角蛋白的保护较弱，可能在原发性男性感染中起重要作用。这些发现为流行病学上观察到的男性包皮环切术的保护作用提供了一个可能的解剖学解释。(c)2006年利平科特威廉姆斯&威尔金斯。
Objectives: To study the distribution of HIV-1 receptors and degree of keratinization in the human penis.Design: Formalin-fixed penises were obtained from nine uncircumcised cadavers. Foreskins were obtained from 21 healthy adult men undergoing elective circumcision for social reasons. Uncircumcised penises were obtained within 24 h of death from eight men. All tissues were stained for keratin and HIV-1 receptors.Methods: Penises from nine formalin fixed cadavers aged 64-80 years were obtained from the Department of Anatomy, University of Melbourne. Foreskins were obtained from 21 men aged 18-64 years following circumcision performed at either the Freemason's or Mercy Private Hospitals, Melbourne, Australia. Fresh penile necropsy specimens from eight uncircumcised men aged 23-63 years were obtained from the Victorian Institute of Forensic Medicine, Melbourne. The degree of keratinization was scored, and the distribution of HIV-1 susceptible cells was mapped in the glans penis, penile urethra, urethral meatus, frenulum and foreskin.Results: Cells with HIV-1 receptors were present in all penile epithelia, but Langerhans' cells were most superficial in the inner foreskin and frenulum. The inner foreskin had a significantly thinner keratin layer (1.8 +/- 0.1 units), than the outerforeskin (3.3 +/- 0.1), or glans penis (3.3 +/- 0.2), P < 0.05.Conclusions: Superficial Langerhans' cells on the inner aspect of the foreskin and frenulum are poorly protected by keratin and thus could play an important role in primary male infection. These findings provide a possible anatomical explanation for the epidemiologically observed protective effect of male circumcision. (c) 2006 Lippincott Williams & Wilkins.