Conservation and divergence of ancestral AGAMOUS/SEEDSTICK subfamily genes from the basal angiosperm Magnolia wufengensis
Conservation and divergence of ancestral AGAMOUS/SEEDSTICK subfamily genes from the basal angiosperm Magnolia wufengensis
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基底被子植物五峰木兰（木兰科）祖先 AGAMOUS/SEEDSTICK 亚家族基因的表达、蛋白质与蛋白质 1 相互作用和功能以及外显子-内含子组织的保守性和分歧。
DOI：
10.1093/treephys/tpz091
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发表时间：
2020-01-01
期刊：
影响因子：
4
通讯作者：
Chen, Faju
中科院分区：
文献类型：
--
作者：
Ma, Jiang;Deng, Shixin;Chen, Faju
AGAMOUS/SEEDSTICK（AG/STK）亚家族基因在植物生殖发育中起着重要作用。然而，目前对AG/STK亚家族基因的研究主要局限于核心双子叶植物和禾本科植物，对AG/STK亚家族基因的外显子-内含子结构、表达模式、蛋白质相互作用模式和功能等方面的研究尚不清楚。为了确定这些，我们分离AG/STK亚家族基因（MawuAG 1，MawuAG 2和MawuSTK）从木本基部被子植物木兰wufengensis（木兰科）。MawuSTK可能起源于现存被子植物分化之前的基因重复事件，MawuAG 1和MawuAG 2可能起源于木兰科和樟科分化之前的基因重复事件。基因复制导致其表达和相互作用模式明显多样化。结果表明，在雄蕊和心皮中的表达可能代表了AG谱系基因的祖先表达谱，STK样基因在雄蕊中的表达可能在STK谱系出现后不久即消失。此外，AG/STK亚家族蛋白可能在SEP亚家族出现后立即与SEPALLATA（SEP）亚家族蛋白建立了相互作用;然而，它们与APETALA 1/FRUITFULL亚家族蛋白或它们本身的相互作用不同于单子叶植物和基部和核心真双子叶植物中发现的相互作用。MawuAG 1在雄蕊、心皮和胚珠身份的决定中起着高度保守的作用，而MawuAG 2和MawuSTK的功能多样性则是由基因重复引起的。此外，我们还研究了AG/STK亚家族外显子-内含子结构变化的进化历史，首次揭示了euAG和PLE亚家族中一种新的剪接-受体模式（GUU-AU）和N-末端延伸的趋同进化。这些结果进一步推进了我们对AG/STK亚家族祖先基因的遗传、外显子-内含子结构、表达和相互作用模式以及功能的理解，并为基因复制在AG/STK亚家族的扩展和进化中的重要性提供了强有力的证据。
AGAMOUS/SEEDSTICK (AG/STK) subfamily genes play crucial roles in the reproductive development of plants. However, most of our current knowledge of AG/STK subfamily genes is restricted to core eudicots and grasses, and the knowledge of ancestral exon-intron structures, expression patterns, protein-protein interaction patterns and functions of AG/STK subfamily genes remains unclear. To determine these, we isolated AG/STK subfamily genes (MawuAG1, MawuAG2 and MawuSTK) from a woody basal angiosperm Magnolia wufengensis (Magnoliaceae). MawuSTK arose from the gene duplication event occurring before the diversification of extant angiosperms, and MawuAG1 and MawuAG2 may result from a gene duplication event occurring before the divergence of Magnoliaceae and Lauraceae. Gene duplication led to apparent diversification in their expression and interaction patterns. It revealed that expression in both stamens and carpels likely represents the ancestral expression profiles of AG lineage genes, and expression of STK-like genes in stamens may have been lost soon after the appearance of the STK lineage. Moreover, AG/STK subfamily proteins may have immediately established interactions with the SEPALLATA (SEP) subfamily proteins following the emergence of the SEP subfamily; however, their interactions with the APETALA1/FRUITFULL subfamily proteins or themselves differ from those found in monocots and basal and core eudicots. MawuAG1 plays highly conserved roles in the determinacy of stamen, carpel and ovule identity, while gene duplication contributed to the functional diversification of MawuAG2 and MawuSTK. In addition, we investigated the evolutionary history of exon-intron structural changes of the AG/STK subfamily, and a novel splice-acceptor mode (GUU-AU) and the convergent evolution of N-terminal extension in the euAG and PLE subclades were revealed for the first time. These results further advance our understanding of ancestral AG/STK subfamily genes in terms of phylogeny, exon-intron structures, expression and interaction patterns, and functions, and provide strong evidence for the significance of gene duplication in the expansion and evolution of the AG/STK subfamily.