Comparison of small biopsy specimens and surgical specimens for the detection of EGFR mutations and EML4-ALK in non-small-cell lung cancer.
Comparison of small biopsy specimens and surgical specimens for the detection of EGFR mutations and EML4-ALK in non-small-cell lung cancer.
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小活检标本与手术标本检测非小细胞肺癌EGFR突变和EML4-ALK的比较
DOI：
10.18632/oncotarget.10011
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发表时间：
2016-09-13
期刊：
影响因子：
--
通讯作者：
Tao Y
中科院分区：
文献类型：
--
作者：
Xiao D;Lu C;Zhu W;He Q;Li Y;Fu C;Zhou J;Liu S;Tao Y
表皮生长因子受体（EGFR）突变和间变性淋巴瘤激酶（ALK）融合基因代表了与非小细胞肺癌（NSCLC）相关的新型癌基因。确定在小活检标本或手术标本中检测EGFR突变和ALK融合基因的可行性。在721例NSCLC患者中，共有305例EGFR突变阳性（42.3%）。EGFR在女性中的突变率明显高于男性。组织学上，腺癌的EGFR突变率明显高于鳞状细胞癌。手术标本（42.1%）与小活检标本（42.4%）EGFR突变率无差异，说明手术标本与小活检标本中EGFR突变率无差异。在385例NSCLC患者中，EML4-ALK阳性26例（6.8%）。然而，11.7%的手术标本eml4 - alk阳性，而在小活检标本中阳性比例仅为4.7%，说明手术标本中eml4 - alk阳性率明显高于小活检标本。在小活检标本中检测EGFR基因突变是可行的，筛选小活检标本中EML4-ALK的表达可用于指导临床治疗。
Epidermal growth factor receptor (EGFR) mutations and anaplastic lymphoma kinase (ALK) fusion genes represent novel oncogenes that are associated with non–small-cell lung cancers (NSCLC). The feasibility of detecting EGFR mutations and ALK fusion genes in small biopsy specimens or surgical specimens was determined. Of the 721 NSCLC patients, a total of 305 cases were positive for EGFR mutations (42.3%). The rate of EGFR mutations in women was significantly higher than that in men. Histologically, the EGFR mutation rate in adenocarcinomas was significantly higher than that in squamous cell carcinomas. No difference in the EGFR mutation rate was observed between surgical specimens (42.1%) and small biopsy specimens (42.4%), which indicated that the EGFR mutation ratios in surgical specimens and small biopsy specimens were not different. In 385 NSCLC patients, 26 cases were positive for EML4-ALK (6.8%). However, 11.7% of the surgical specimens were EML4-ALK-positive, whereas the positive proportion in the small biopsy specimens was only 4.7%, which indicated that EML4-ALK-positive rate in the surgical specimens was significantly higher than that in the small biopsy specimens. Detection of EGFR gene mutations was feasible in small biopsy specimens, and screening for EML4-ALK expression in small biopsy specimens can be used to guide clinical treatments.