Using expression profiling to understand the effects of chronic cadmium exposure on MCF-7 breast cancer cells.
Using expression profiling to understand the effects of chronic cadmium exposure on MCF-7 breast cancer cells.
复制标题
DOI：
10.1371/journal.pone.0084646
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Louie MC
中科院分区：
文献类型：
--
作者：
Lubovac-Pilav Z;Borràs DM;Ponce E;Louie MC
镉是一种已知的金属雌激素，可以激活雌激素受体，促进乳腺癌细胞的生长。以前的研究表明，镉与更多的恶性肿瘤的发生有关；然而，这种镉诱导的恶性肿瘤背后的分子机制仍然不清楚。利用乳腺癌细胞暴露于镉6个月以上的克隆性细胞系(MCF-7-CD_4、-CD_6、-CD_(7)、-CD_8和CD_(12))，本研究旨在确定与慢性镉暴露相关的基因表达特征。我们的结果表明，长期接触镉不仅会导致基因放松调控，而且实际上会导致一种独特的表达模式。镉暴露细胞中失控的基因参与多种生物学过程(如细胞生长、细胞凋亡等)。以及分子功能(如镉/金属离子结合、转录因子活性等)。系统聚类法表明，这五个克隆镉细胞系共享乳腺癌相关基因的共同基因表达特征，明显区分了对照细胞和镉暴露细胞。这项研究的结果对镉对乳腺癌的细胞和分子影响提供了深入的见解，并强调了研究慢性镉暴露作为促进乳腺癌进展的可能机制之一的重要性。
Cadmium is a metalloestrogen known to activate the estrogen receptor and promote breast cancer cell growth. Previous studies have implicated cadmium in the development of more malignant tumors; however the molecular mechanisms behind this cadmium-induced malignancy remain elusive. Using clonal cell lines derived from exposing breast cancer cells to cadmium for over 6 months (MCF-7-Cd4, -Cd6, -Cd7, -Cd8 and -Cd12), this study aims to identify gene expression signatures associated with chronic cadmium exposure. Our results demonstrate that prolonged cadmium exposure does not merely result in the deregulation of genes but actually leads to a distinctive expression profile. The genes deregulated in cadmium-exposed cells are involved in multiple biological processes (i.e. cell growth, apoptosis, etc.) and molecular functions (i.e. cadmium/metal ion binding, transcription factor activity, etc.). Hierarchical clustering demonstrates that the five clonal cadmium cell lines share a common gene expression signature of breast cancer associated genes, clearly differentiating control cells from cadmium exposed cells. The results presented in this study offer insights into the cellular and molecular impacts of cadmium on breast cancer and emphasize the importance of studying chronic cadmium exposure as one possible mechanism of promoting breast cancer progression.
登录
查看更多内容
影响因子：
2.7
作者：
Garrett SH;Clarke K;Sens DA;Deng Y;Somji S;Zhang KK
通讯作者：
Zhang KK
影响因子：
10.4
作者：
Benbrahim-Tallaa L;Tokar EJ;Diwan BA;Dill AL;Coppin JF;Waalkes MP
通讯作者：
Waalkes MP
影响因子：
3.5
作者：
Hoefer, Nicola;Diel, Patrick;Degen, Gisela H.
通讯作者：
Degen, Gisela H.
影响因子：
14.9
作者：
Draghici, S;Khatri, P;Tainsky, MA
通讯作者：
Tainsky, MA
影响因子：
10.3
作者：
Alonso-Gonzalez, C.;Gonzalez, A.;Mediavilla, M. D.
通讯作者：
Mediavilla, M. D.