Cover Picture: Two‐Dimensional Multiarray Formation of Hepatocyte Spheroids on a Microfabricated PEG‐Brush Surface (ChemBioChem 6/2004)
Cover Picture: Two‐Dimensional Multiarray Formation of Hepatocyte Spheroids on a Microfabricated PEG‐Brush Surface (ChemBioChem 6/2004)
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封面图片：微加工 PEG 刷表面上肝细胞球体的二维多阵列形成 (ChemBioChem 6/2004)
DOI：
10.1002/cbic.200490024
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发表时间：
2004
期刊：
影响因子：
3.2
通讯作者：
K. Kataoka
中科院分区：
文献类型：
--
作者：
H. Otsuka;A. Hirano;Y. Nagasaki;T. Okano;Y. Horiike;K. Kataoka
封面图片显示了一种简单有效的方法，通过表面 PEG 处理和随后的等离子蚀刻微加工相结合，构建 10 000 个肝细胞异质球体的二维微阵列，其下方铺有内皮细胞。微图案化的 PEG 处理区域可排斥蛋白质，从而抑制细胞粘附，而由 PEG 处理区域 (l=100μm) 分隔的玻璃圆形域 (Ø=100μm) 则提供细胞粘附区域。这种球体阵列作为基于组织和细胞的生物传感器的平台非常有用，可以在各种情况下用作动物实验的替代方案。有关更多详细信息，请参阅 Kataoka 等人的文章。上页。 850 及以下
The cover picture showsa simple and effective way to construct a 2D microarray of 10 000 hepatocyte heterospheroids, underlaid with endothelial cells, through a combination of surface PEG treatment and subsequent microfabrication of plasma etching. The micropatterned PEG‐treated region works to repel proteins and consequently inhibits cell adhesion, while the glass circular domains (Ø=100 μm), separated by PEG‐treated regions (l=100 μm), provide cell adhesive regions. This spheroid array is very useful as a platform for tissue and cell‐based biosensors, which can be used as an alternative to animal experiments in a variety of situations. For more details, see the article by Kataoka et al. on p. 850 ff.