Fundamental aspects of the interaction of propidium diiodide with nuclei acids studied in a model system of polyacrylamide films.
Fundamental aspects of the interaction of propidium diiodide with nuclei acids studied in a model system of polyacrylamide films.
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在聚丙烯酰胺膜模型系统中研究了二碘化丙啶与核酸相互作用的基本方面。
DOI：
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发表时间：
1981
影响因子：
3.2
通讯作者：
G. Westerneng
中科院分区：
文献类型：
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作者：
J. Tas;G. Westerneng
定性和定量方面的荧光二碘化丙啶（PI）染色方法进行了研究与模型膜的聚丙烯酰胺凝胶掺入DNA，RNA，和其他大分子化合物。PI被发现特异性地结合到DNA和RNA，最有可能通过插入到双链区域。PI染色后，蛋白质、糖原和糖胺聚糖未显示荧光。染料结合和分化的最佳条件已经确定。核酸-PI复合物的稳定性，如存在于模型膜中，显示在蒸馏水中非常高，而在离子介质中迅速发生解离。结合PI的荧光强度与模型膜的厚度和DNA或RNA的掺入量之间存在线性关系。组蛋白与DNA离子结合的存在对荧光PI结合能力没有任何明显的影响。
Qualitative and quantitative aspects of the fluorescent propidium diiodide (PI) staining method have been investigated with model films of polyacrylamide gel incorporated with DNA, RNA, and other macromolecular compounds. PI was found to bind specifically to DNA and RNA, most probably by intercalation into double-stranded regions. Proteins, glycogen, and glycosaminoglycans did not show fluorescence after PI staining. Optimal conditions for dye binding and differentiation have been defined. The stability of nucleic acid-PI complexes, as present in model films, was shown to be very high in distilled water, while dissociation rapidly occurred in ionic media. Linear relationships were found between the fluorescence intensity of bound PI and both the thickness of the model films and the amount of DNA or RNA incorporated. The presence of histone protein bound ionically to DNA did not influence the fluorescent PI binding ability in any appreciable amount.