Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans.
Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans.
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Bcr1 和 CFEM 蛋白在近平滑念珠菌和白色念珠菌中的保守和不同作用。
DOI：
10.1371/journal.pone.0028151
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Butler G
中科院分区：
文献类型：
--
作者：
Ding C;Vidanes GM;Maguire SL;Guida A;Synnott JM;Andes DR;Butler G
近缘念珠菌是一种病原性真菌，是医院获得性感染的主要原因，主要是由于留置医疗器械上的生物膜生长所致。它与白色念珠菌有关，白色念珠菌仍然是人类念珠菌病最常见的原因。转录因子Bcr1是近缘念珠菌和白色念珠菌体外生物膜形成的重要调节因子。我们在这里展示了在大鼠导管模型中，如白色念珠菌的体内生物膜发育所需要的近缘念珠菌Bcr1。通过比较两个物种中bcr1缺失的转录图谱，我们发现CFEM家族的表达调控是保守的。在白色念珠菌中，五种CFEM细胞壁蛋白中的三种(Rbt5、Pga7和Csa1)与生物膜的形成和从血红素中获取铁有关，这是一个重要的毒力特征。在巴氏梭菌病中，CFEM家族经历了7个成员的扩张。三个基因(CFEM2、CFEM3和CFEM6)的表达依赖于Bcr1，并在低铁条件下诱导。这三家公司都参与了从亚铁血红素中获取铁的过程。然而，这三个cfem基因的缺失对巴氏弧菌生物膜的形成没有影响。我们的数据表明，CFEM家族在铁获取中的作用在白色念珠菌和近缘念珠菌之间是保守的，但它们在生物膜形成中的作用不是这样的。
Candida parapsilosis is a pathogenic fungus that is major cause of hospital-acquired infection, predominantly due to growth as biofilms on indwelling medical devices. It is related to Candida albicans, which remains the most common cause of candidiasis disease in humans. The transcription factor Bcr1 is an important regulator of biofilm formation in vitro in both C. parapsilosis and C. albicans. We show here that C. parapsilosis Bcr1 is required for in vivo biofilm development in a rat catheter model, like C. albicans. By comparing the transcription profiles of a bcr1 deletion in both species we found that regulation of expression of the CFEM family is conserved. In C. albicans, three of the five CFEM cell wall proteins (Rbt5, Pga7 and Csa1) are associated with both biofilm formation and acquisition of iron from heme, which is an important virulence characteristic. In C. parapsilosis, the CFEM family has undergone an expansion to 7 members. Expression of three genes (CFEM2, CFEM3, and CFEM6) is dependent on Bcr1, and is induced in low iron conditions. All three are involved in the acquisition of iron from heme. However, deletion of the three CFEM genes has no effect on biofilm formation in C. parapsilosis. Our data suggest that the role of the CFEM family in iron acquisition is conserved between C. albicans and C. parapsilosis, but their role in biofilm formation is not.
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影响因子：
30.3
作者：
Chen C;Pande K;French SD;Tuch BB;Noble SM
通讯作者：
Noble SM
影响因子：
--
作者：
Baek, Yong-Un;Li, Mingchun;Davis, Dana A.
通讯作者：
Davis, Dana A.
影响因子：
4.4
作者：
Fitzpatrick, David A.;O'Gaora, Peadar;Butler, Geraldine
通讯作者：
Butler, Geraldine
影响因子：
9.4
作者：
Clark, TA;Slavinski, SA;Hajjeh, RA
通讯作者：
Hajjeh, RA
影响因子：
3.1
作者：
Andes, D;Nett, J;Pitula, A
通讯作者：
Pitula, A