Cytotoxic and endocrine-disrupting potential of atrazine, diazinon, endosulfan, and mancozeb in adrenocortical steroidogenic cells of rainbow trout exposed in vitro
Cytotoxic and endocrine-disrupting potential of atrazine, diazinon, endosulfan, and mancozeb in adrenocortical steroidogenic cells of rainbow trout exposed in vitro
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DOI：
10.1006/taap.2002.9377
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发表时间：
2002-04-15
影响因子：
3.8
通讯作者：
Hontela, A
中科院分区：
文献类型：
--
作者：
Bisson, M;Hontela, A
建立了一种用于检测和定量评估具有干扰肾上腺类固醇合成能力的化学物质的体外生物测定方法，并用于比较四种杀虫剂的细胞毒性和内分泌干扰潜力。将酶分散的虹鱼(Oncorhynchus Mykis)肾上腺皮质细胞暴露于阿特拉津、二氮磷、硫丹和代森锰锌，测定ACTH或二丁酰cAMP(DBcAMP)对肾上腺皮质细胞的皮质醇分泌和细胞活力。确定了受试农药的有效浓度、半数致死浓度和半数致死浓度，以及半数致死浓度和半数致死浓度，以及半数致死浓度和半数致死浓度。杀虫剂的等级如下：EC50、硫丹和二氮磷
An in vitro bioassay for detection and quantitative assessment of chemicals with the capacity to disrupt adrenal steroidogenesis has been developed and used to compare the cytotoxic and endocrine-disrupting potential of four pesticides. Enzymatically dispersed adrenocortical cells of rainbow trout (Oncorhynchus mykiss) were exposed in vitro to atrazine, diazinon,endosulfan, and mancozeb, and cortisol secretion in response to ACTH or dibutyryl-cAMP (dbcAMP) and cell viability were determined. The effective concentration, EC50 (concentration that inhibits cortisol secretion by 50%), the median lethal concentration, LC50 (concentration that kills 50% of the cells), and the LC50/EC50 ratio were established for the test pesticides. The pesticides were ranked as follows: EC50, endosulfan < diazinon