Evaluation of mammalian cell adhesion on surface-modified porous silicon
Evaluation of mammalian cell adhesion on surface-modified porous silicon
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DOI：
10.1016/j.biomaterials.2006.04.015
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发表时间：
2006-09-01
期刊：
影响因子：
14
通讯作者：
Voelcker, Nicolas H.
中科院分区：
文献类型：
--
作者：
Low, Suet Peng;Williams, Keryn A.;Voelcker, Nicolas H.
多孔硅是一种无毒、可生物降解的生物材料，具有广阔的应用前景。表面改性可以控制降解速度，还可以赋予促进细胞黏附的性能。在本研究中，我们对多孔硅表面进行了臭氧氧化、硅烷化或胶原或血清包覆的表面修饰。对于每个表面，用原子力显微镜表征表面形貌，用水接触角测量润湿性，用干涉反射光谱表征水溶液中的降解，用傅里叶变换红外光谱表征表面化学。研究了大鼠嗜铬细胞瘤(PC12)和人晶状体上皮细胞在这些表面上的黏附情况。细胞在细胞表面孵育4小时和24小时，用荧光活体染色和细胞计数来确定细胞的黏附特性。胶原蛋白涂层和氨基硅烷化的多孔硅促进了两种细胞系的细胞附着，而细胞在臭氧氧化和聚乙二醇硅烷化的表面上附着较差。我们发现两种细胞株在不同的时间点在不同的表面上有不同的黏附特性。此外，还研究了活性检测方法阿拉玛蓝(氧化还原法)和中性红(活性细胞摄取法)在多孔硅上的应用。我们揭示了某些Resazurin(阿拉马蓝)、溶酶体掺入试验(中性红)和多孔硅之间的不相容。(C)2006爱思唯尔有限公司。保留所有权利。
Porous silicon is a promising biomaterial that is non-toxic and biodegradable. Surface modification can offer control over the degradation rate and can also impart properties that promote cell adhesion. In this study, we modified the surface of porous silicon surface by ozone oxidation, silanisation or coating with collagen or serum. For each surface, topography was characterised using atomic force microscopy, wettability by water contact angle measurements, degradation in aqueous buffer by interferometric reflectance spectroscopy and surface chemistry by Fourier-transform infrared spectroscopy. The adhesion of rat pheochromocytoma (PC12) and human lens epithelial cells to these surfaces was investigated. Cells were incubated on the surfaces for 4 and 24 h, and adhesion characteristics were determined by using a fluorescent vital stain and cell counts. Collagen coated and amino silanised porous silicon promoted cell attachment for both cell lines whereas cells attached poorly to ozone oxidised and polyethylene glycol silanised surfaces. We showed that the two cell lines had different adhesion characteristics on the various surfaces at different time points. The use of the vitality assays Alamar Blue (redox based assay) and neutral red (active cellular uptake assay) with porous silicon was also investigated. We reveal incompatibilities between certain resazurin (Alamar Blue), lysosomal incorporation assays (neutral red) and porous silicon. (c) 2006 Elsevier Ltd. All rights reserved.