Effect of alginate composition and gelling cation on micro-bead swelling
Effect of alginate composition and gelling cation on micro-bead swelling
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DOI：
10.1080/02652040500286144
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发表时间：
2006-02-01
影响因子：
3.9
通讯作者：
Opara, Emmanuel C.
中科院分区：
文献类型：
--
作者：
Darrabie, Marcus D.;Kendall, William F.;Opara, Emmanuel C.
Purpose. The purpose of this study was to determine the roles of alginate composition and gelling cations on bead swelling, which affects its durability.Method. Using a 2-channel droplet generator, microspheres were generated with 1.5% solutions of low viscosity high-mannuronic acid (LVM), medium viscosity high-mannuronic acid (MVM), low viscosity high-guluronic acid (LVG) and medium viscosity high-guluronic acid (MVG) alginate. They were gelled by cross-linking with 1.1% solution of either BaCl2 or CaCl2. The diameters of the micro-beads were measured and recorded on day 0. The micro-beads were subsequently washed and incubated in saline at 37 degrees C for 2 weeks with size assessment every 2 days. The data were normalized by calculation of the percentage change from control (day 0) for all groups of micro-beads.Results. Diameters of all beads were between 550 and 700 pm on day 0. Viscosity had no effect on swelling of Ba++- and Ca++-alginate micro-beads. Ca++-alginate micro-beads were more prone to swelling than the corresponding Ba++-alginate beads. High G-Ba++ beads had only a modest increase in size over time, in contrast to the high M-Ba++.Conclusion. Alginate composition and the gelling cation have significant effects on bead swelling.
