IDENTITY OF THE ENDOSYMBIONT OF PERIDINIUM FOLIACEUM (PYRROPHYTA): ANALYSIS OF THE rbcLS OPERON 1
IDENTITY OF THE ENDOSYMBIONT OF PERIDINIUM FOLIACEUM (PYRROPHYTA): ANALYSIS OF THE rbcLS OPERON 1
复制标题
叶甲藻 (Pyrrophyta) 内共生体的身份：rbcLS 操纵子 1 的分析
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
Andrea M. Schmieg
中科院分区：
文献类型：
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作者：
J. Chesnick;C. Morden;Andrea M. Schmieg
现存的有色藻类被认为是通过无色原生生物吞噬光合细菌而产生的。甲藻Peridinium foliaceum（Stein）Biecheler含有一个减少的叶绿素c-含内共生体，因此，代表了一个进化的中间阶段，在叶绿体的建立。虽然共生体与现存藻类物种的确切系统发育关系尚不清楚，但有人认为P. foliaceum共生体是硅藻或金藻。确定P. foliaceum共生体的最近生活亲属将提供一个自由生活的模型系统，可以与光合共生体进行比较。对叶状囊藻共生体的rbcL和rbcS（编码1，5-二磷酸核酮糖羧化酶/加氧酶的大小亚基）进行核苷酸序列分析，以了解其身份。从叶绿体DNA文库中克隆的限制性片段进行筛选，并对编码rbcLS操纵子的克隆进行测序。对每个基因进行简约系统发育分析。我们的数据有力地表明，共生体起源于光合硅藻。
Extant chromophytic algae have been suggested to have originated via the engulfment of a photo synthetic alga by a colorless protist. The dinoflagellate Peridinium foliaceum (Stein) Biecheler contains a reduced chlorophyll c–containing endosymbiont and, thus, represents an evolutionary intermediate stage in the establishment of chloroplasts. Although the exact phylogenetic relationship of the symbiont to extant algal species is unknown, it had been suggested that the P. foliaceum symbiont was either a diatom or a chrysophyte. Identification of the closest living relative of the P. foliaceum symbiont would provide a free‐living model system with which the photosynthetic symbiont could be compared. Nucleotide sequence analysis of rbcL and rbcS (encoding the large and small subunits ofribulose‐1,5‐bisphosphate carboxylase/oxygenase) by the P. foliaceum symbiont was performed to provide insights into its identity. Cloned restriction fragments from a chloroplast DNA library were screened, and clones encoding the rbcLS operon were sequenced. Parsimony phylogenetic analysis was performed for each gene. Our data strongly suggest that the symbiont originated from a photosynthetic diatom.
影响因子：
56.9
作者：
MORSE, D;SALOIS, P;HASTINGS, JW
通讯作者：
HASTINGS, JW