Water impacts nutrient dose responses genome-wide to affect crop production
Water impacts nutrient dose responses genome-wide to affect crop production
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DOI：
10.1038/s41467-019-09287-7
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发表时间：
2019-03
影响因子：
16.6
通讯作者：
Joseph Swift;Mark Adame;D. Tranchina;A. Henry;G. Coruzzi
中科院分区：
文献类型：
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作者：
Joseph Swift;Mark Adame;D. Tranchina;A. Henry;G. Coruzzi
营养剂量的变化对基因表达和发育有显著影响。一个悬而未决的问题是，生物体是否会对绝对营养量（摩尔）与其在水中的浓度（摩尔浓度）的变化做出反应。这个问题与植物特别相关，因为土壤干燥可以改变养分浓度，而不会改变其绝对量。为了比较量与浓度的影响，我们将水稻暴露于在一系列组合中改变氮（N）和水（W）剂量的因子矩阵，并量化转录组和表型响应。使用线性模型，我们确定了不同的剂量反应，无论是N-摩尔，W-体积，N-摩尔浓度（N/W），或其协同作用（N×W）。重要的是，在重复的田间试验中，其表达模式最好由幼苗中的N-剂量和W相互作用（N/WorN×W）解释的基因与作物结果相关。这种N × W响应基因可能有助于未来开发适应日益干旱，低养分土壤的作物。
Changes in nutrient dose have dramatic effects on gene expression and development. One outstanding question is whether organisms respond to changes in absolute nutrient amount (moles) vs. its concentration in water (molarity). This question is particularly relevant to plants, as soil drying can alter nutrient concentration, without changing its absolute amount. To compare the effects of amount vs. concentration, we expose rice to a factorial matrix varying the dose of nitrogen (N) and water (W) over a range of combinations, and quantify transcriptome and phenotype responses. Using linear models, we identify distinct dose responses to eitherN-moles,W-volume,N-molarity (N/W), or their synergistic interaction (N×W). Importantly, genes whose expression patterns are best explained by N-dose and W interactions (N/WorN×W) in seedlings are associated with crop outcomes in replicated field trials. Such N-by-W responsive genes may assist future efforts to develop crops resilient to increasingly arid, low nutrient soils.