A high-speed atomic force microscope for studying biological macromolecules in action
A high-speed atomic force microscope for studying biological macromolecules in action
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DOI：
10.1143/jjap.41.4851
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发表时间：
2002-07-01
期刊：
影响因子：
--
通讯作者：
Toda, A
中科院分区：
文献类型：
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作者：
Ando, T;Kodera, N;Toda, A
原子力显微镜（AFM）是一种强大的工具，用于在溶液中对基底上的生物分子进行成像。然而，AFM没有有效的时间轴;商业上可用的AFM需要几分钟才能捕获图像，但许多有趣的生物过程以更高的速率发生。因此，我们使用AFM所能观察到的仅限于静止的分子，或那些移动非常缓慢的分子。我们试图显著提高AFM的扫描速度，以便将来可以用于研究生物分子的动力学行为。为此，我们开发了各种针对高速扫描进行优化的设备。结合这些设备已经产生了一个AFM，可以在80 ms内捕获100 x 100像素的图像，从而生成一个由水溶液中样品的许多连续图像组成的电影。这是通过成像肌球蛋白V分子在云母上移动，在溶液中证明。
The atomic force microscope (AFM) is a powerful tool for imaging biological molecules on a substrate, in solution. However, there is no effective time axis with AFM; commercially available AFMs require minutes to capture an image, but many interesting biological processes occur at a much higher rate. Hence, what we can observe using the AFM is limited to stationary molecules, or those moving very slowly. We sought to increase markedly the scan speed of the AFM, so that in the future it can be used to study the dynamic behavior of biomolecules. For this purpose, we have developed various devices optimized for high-speed scanning. Combining these devices has produced an AFM that can capture a 100 x 100 pixel image within 80 ms, thus generating a movie consisting of many successive images of a sample in aqueous solution. This is demonstrated by imaging myosin V molecules moving on mica, in solution.