Generation of stella-GFP transgenic mice:: A novel tool to study germ cell development
Generation of stella-GFP transgenic mice:: A novel tool to study germ cell development
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DOI：
10.1002/gene.20187
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发表时间：
2006-02-01
期刊：
影响因子：
1.5
通讯作者：
Surani, MA
中科院分区：
文献类型：
--
作者：
Payer, B;Lopes, SMCD;Surani, MA
生殖细胞和多能胚胎干细胞（ES）之间的关系以及从ES细胞产生原始生殖细胞（PGCs）的方法尤其令人感兴趣。这些实验的一个关键要求是能够使用报告基因等方法明确地检测PGCs。目前可用的转基因报告基因在PGCs的早期发育阶段并未显示出排他性表达。在这里，我们描述了stella的种系限制性表达的使用，这是目前最好的PGCs标记基因。我们生成了两个stella-GFP报告基因，并表明这两个转基因在PGCs中表达的时间和特异性方面都超过了其他报告基因。此外，我们证明了stella-GFP在胚胎干细胞衍生PGCs过程中的作用。
The relationship between germ cells and pluripotent embryonic stem (ES) cells is of particular interest, together with approaches to generate primordial germ cell (PGCs) from ES cells. A critical requirement in these experiments is the ability to unambiguously detect PGCs with the use of, for example, reporter genes. The currently available transgenic reporters do not show exclusive expression in PGCs at their earliest developmental stages. Here we describe the use of germline-restricted expression of stella, which is currently the best marker gene for PGCs. We generated two stella-GFP reporters and show that both transgenes surpass other reporters in terms of timing and specificity of expression in PGCs. Additionally, we demonstrate the usefulness of stella-GFP during the derivation of PGCs from ES cells.