Nonomuraea lycopersici sp nov., isolated from the root of tomato plants (Solanum lycopersicum L.)
Nonomuraea lycopersici sp nov., isolated from the root of tomato plants (Solanum lycopersicum L.)
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Nonomuraea lycopersici sp nov.，从番茄植物 (Solanum lycopersicum L.) 的根中分离出来
DOI：
10.1007/s10482-017-1012-6
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发表时间：
2018
影响因子：
2.6
通讯作者：
Xiang WS
中科院分区：
文献类型：
--
作者：
Zheng Weiwei;Zhao Junwei;Li Dongmei;Jiang Hao;Han Liyuan;Zhao Xueli;Chen Yufei;Wang XJ;Xiang WS
从河南省登封市番茄植株（Solanum lycopersicumL.）根中分离到两株新菌株。16S rRNA基因序列的系统发育分析表明，分离株NEAU-DE8(1)和NEAU-HE1(2)亲缘关系较近（相似度为99.6%），与toNonomuraea zeaeNEAU-ND5T亲缘关系较近（相似度为98.3%），与genenzanensisdsm100948t亲缘关系较近（相似度为98.3%）。基于16S rRNA基因序列构建的系统发育树显示，这两个菌株都属于n。[au - n5]在包含非蝇属成员的分支中。此外，包括主要的甲基萘醌类、脂肪酸组成和磷脂谱在内的关键化学分类特性也证实了这两株菌株属于诺莫菌属。然而，DNA-DNA杂交数据以及生理和形态特征表明，这两个分离株可以很容易地与它们的近亲区分开来。因此，根据这些表型和基因型数据，我们认为菌株NEAU-DE8(1)和NEAU-HE1(2)代表了一种新的noonomuraea属，并将其命名为enonomuraea lycopersicisp。11月被提议。型应变为NEAU-DE8(1)T（= CGMCC 4.7412T= DSM 104642T）。
Two novel bacterial strains were isolated from the roots of tomato plants (Solanum lycopersicumL.) from Dengfeng, Henan Province, China. Phylogenetic analysis of 16S rRNA gene sequences showed that the isolates NEAU-DE8(1)Tand NEAU-HE1(2) are closely related to one another (99.6% similarity) and are closely relate toNonomuraea zeaeNEAU-ND5T(98.3, 98.3%) andNonomuraea gerenzanensisDSM 100948T(98.3, 98.0%). Phylogenetic trees constructed based on 16S rRNA gene sequences revealed that both strains grouped withN. zeaeNEAU-ND5Twithin the clade comprising the members of the genusNonomuraea.Moreover, key chemotaxonomic properties including major menaquinones, fatty acid composition and phospholipid profiles also confirmed the affiliation of the two strains to the genusNonomuraea. However, DNA–DNA hybridization data, together with physiological and morphological properties, showed that two isolates could be readily distinguished from their close phylogenetic relatives. Thus, on the basis of these phenotypic and genotypic data, strains NEAU-DE8(1)Tand NEAU-HE1(2) are concluded to represent a novel species of the genusNonomuraea, for which the nameNonomuraea lycopersicisp. nov. is proposed. The type strain is NEAU-DE8(1)T(= CGMCC 4.7412T= DSM 104642T).