Effect of electro-acupuncture on gene expression in heart of rats with stress-induced pre-hypertension based on gene chip technology.
Effect of electro-acupuncture on gene expression in heart of rats with stress-induced pre-hypertension based on gene chip technology.
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DOI：
10.1016/s0254-6272(15)30099-6
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发表时间：
2015-06
影响因子：
2.6
通讯作者：
G. Yan;Xie Xiaojia;G. Chang-qing;Wang Zhaoyang;L. Qing-guo
中科院分区：
文献类型：
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作者：
G. Yan;Xie Xiaojia;G. Chang-qing;Wang Zhaoyang;L. Qing-guo
目的探讨电针对应激性高血压前期大鼠心脏基因表达的影响，并试图通过基因芯片技术揭示其生物学机制。方法选取Wistar雄性大鼠27只，随机分为3组。采用足部电刺激结合产生的噪声制备应激性高血压大鼠模型。造模周期为14 d，模型+ EA组在造模过程中给予EA干预。采用大鼠基因2.0传感靶阵列技术测定基因表达谱，筛选到的关键基因采用实时定量聚合酶链反应（RT-PCR）法进行验证。结果与空白对照组比较，模型组有390个基因发生改变；与模型对照组相比，模型+EA组共改变330个基因。基因功能的显著性分析表明，差异表达的基因是参与生物过程、分子功能和细胞成分的基因。筛选的关键基因RT-PCR结果与基因芯片试验结果一致。结论ea可显著降低应激性高血压前期大鼠血压，并影响其心脏基因表达谱。与血管平滑肌收缩相关的基因可能参与了EA的降压机制。
ObjectiveTo explore electro-acupuncture's (EA's) effect on gene expression in heart of rats with stress-induced pre-hypertension and try to reveal its biological mechanism based on gene chip technology.MethodsTwenty-seven Wistar male rats were randomly divided into 3 groups. The stress-induced hypertensive rat model was prepared by electric foot-shocks combined with generated noise. Molding cycle lasted for 14 days and EA intervene was applied on rats in model + EA group during model preparation. Rat Gene 2.0 Sense Target Array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction (RT-PCR) method.ResultsCompared with blank control group, 390 genes were changed in model group; compared with model control group, 330 genes were changed in model+EA group. Significance analysis of gene function showed that the differentially expressed genes are those involved in biological process, molecular function and cellular components. RT-PCR result of the screened key genes is consistent with that of gene chip test.ConclutionEA could significantly lower blood pressure of stress-induced pre-hypertension rats and affect its gene expression profile in heart. Genes that related to the contraction of vascular smooth muscle may be involved in EA's anti-hypertensive mechanism.