A genome-wide survey of DNA methylation in hexaploid wheat.
A genome-wide survey of DNA methylation in hexaploid wheat.
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DOI：
10.1186/s13059-015-0838-3
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发表时间：
2015-12-10
期刊：
影响因子：
12.3
通讯作者：
Hall A
中科院分区：
文献类型：
--
作者：
Gardiner LJ;Quinton-Tulloch M;Olohan L;Price J;Hall N;Hall A
DNA甲基化是表观遗传基因表达控制的重要机制，可以在世代之间传递。在这里，我们使用亚硫酸氢钠处理和靶向基因富集来研究异源六倍体小麦三个亚基因组的全基因组甲基化。虽然大多数甲基化在所有三个基因组中是保守的，但我们证明了亚基因组之间以大致相等的比例存在差异甲基化。我们将亚基因组特异性启动子甲基化与表达水平降低相关联，并表明生长温度的改变对甲基化状态的影响很小，确定了一组小但功能相关的甲基化基因。最后，我们通过比较六倍体小麦和其祖先节节麦的D亚基因组来证明甲基化的长期维持。我们发现，三基因组甲基化是高度保守的二倍体小麦祖先，而亚基因组特异性甲基化显示更多的变化。本文的在线版本（doi：10.1186/s13059-015-0838-3）包含补充材料，可供授权用户使用。
DNA methylation is an important mechanism of epigenetic gene expression control that can be passed between generations. Here, we use sodium bisulfite treatment and targeted gene enrichment to study genome-wide methylation across the three sub-genomes of allohexaploid wheat. While the majority of methylation is conserved across all three genomes we demonstrate that differential methylation exists between the sub-genomes in approximately equal proportions. We correlate sub-genome-specific promoter methylation with decreased expression levels and show that altered growing temperature has a small effect on methylation state, identifying a small but functionally relevant set of methylated genes. Finally, we demonstrate long-term methylation maintenance using a comparison between the D sub-genome of hexaploid wheat and its progenitor Aegilops tauschii. We show that tri-genome methylation is highly conserved with the diploid wheat progenitor while sub-genome-specific methylation shows more variation. The online version of this article (doi:10.1186/s13059-015-0838-3) contains supplementary material, which is available to authorized users.