Optical coherence tomography based microangiography for quantitative monitoring of structural and vascular changes in a rat model of acute uveitis in vivo: a preliminary study
Optical coherence tomography based microangiography for quantitative monitoring of structural and vascular changes in a rat model of acute uveitis in vivo: a preliminary study
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DOI：
10.1117/1.jbo.20.1.016015
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发表时间：
2015-01-01
影响因子：
3.5
通讯作者：
Wang, Ruikang K.
中科院分区：
文献类型：
--
作者：
Choi, Woo June;Pepple, Kathryn L.;Wang, Ruikang K.
啮齿动物葡萄膜炎模型对研究人类葡萄膜炎的发病机制和制定适当的治疗策略具有重要意义。在小动物模型中对眼部炎症进行定量监测，为评估葡萄膜炎进展和/或治疗效果提供了客观指标。我们提出了一种新的应用光学相干断层扫描（OCT）和基于OCT的微血管造影（OMAG）的大鼠急性葡萄膜炎模型，该模型是由玻璃体内注射一种杀死的分枝杆菌提取物引起的。OCT/OMAG用于提供注射前（基线）和注射后两天（炎症峰值）大鼠眼睛前段的无创三维成像。与基线图像相比，OCT成像可识别炎症动物前段的特征性结构和血管变化。确定的炎症特征包括前房细胞、角膜水肿、瞳孔膜和虹膜血管扩张。相比之下，类固醇治疗的眼睛与对照组没有显著差异。这些发现与同一只眼睛的组织学评估结果进行了比较。此外，定量测量角膜中央厚度和虹膜血管直径。这项初步研究表明，基于oct的微血管造影有望成为评估和管理体内葡萄膜炎的有用工具。（三）2015年中国光学仪器工程师学会
Uveitis models in rodents are important in the investigation of pathogenesis in human uveitis and the development of appropriate therapeutic strategies for treatment. Quantitative monitoring of ocular inflammation in small animal models provides an objective metric to assess uveitis progression and/or therapeutic effects. We present a new application of optical coherence tomography (OCT) and OCT-based microangiography (OMAG) to a rat model of acute anterior uveitis induced by intravitreal injection of a killed mycobacterial extract. OCT/OMAG is used to provide noninvasive three-dimensional imaging of the anterior segment of the eyes prior to injection (baseline) and two days post-injection (peak inflammation) in rats with and without steroid treatments. OCT imaging identifies characteristic structural and vascular changes in the anterior segment of the inflamed animals when compared to baseline images. Characteristics of inflammation identified include anterior chamber cells, corneal edema, pupillary membranes, and iris vasodilation. In contrast, no significant difference from the control is observed for the steroid-treated eye. These findings are compared with the histology assessment of the same eyes. In addition, quantitative measurements of central corneal thickness and iris vessel diameter are determined. This pilot study demonstrates that OCT-based microangiography promises to be a useful tool for the assessment and management of uveitis in vivo. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)