Stromal progenitor cells promote leukocyte migration through production of stromal-derived growth factor 1α:: a potential mechanism for stromal progenitor cell-mediated enhancement of cellular recruitment to wounds
Stromal progenitor cells promote leukocyte migration through production of stromal-derived growth factor 1α:: a potential mechanism for stromal progenitor cell-mediated enhancement of cellular recruitment to wounds
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DOI：
10.1016/j.jpedsurg.2008.02.043
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发表时间：
2008-06-01
影响因子：
2.4
通讯作者：
Liechty, Kenneth W.
中科院分区：
文献类型：
--
作者：
Badillo, Andrea T.;Zhang, Liping;Liechty, Kenneth W.
背景/目的：基质祖细胞（SPC）增强多种损伤模型中的组织修复。然而，SPC 促进组织修复的机制仍然知之甚少。我们假设 SPC 增强的组织修复部分是由于 SPC 介导的循环细胞募集到组织损伤区域。为了测试这一点，我们在体外检查了白细胞对 SPC 的迁移反应。方法：使用 Transwell 测定系统（Coming，由 Fisher Scientific，匹兹堡，宾夕法尼亚州分销）评估白细胞对 SPC、SPC + 转化生长因子 (TGF)-β 1 或 SPC + AMD3100 的响应。从下室收集上清液，并分析白细胞含量、白细胞活力和基质衍生生长因子 (SDF)-1 α 浓度。结果：与单独培养基相比，基质祖细胞增加了白细胞迁移（450 70 vs 112 +/- 17 细胞/μL；P < .05）。使用 TGF-β 1 进行 SPC 处理后，白细胞迁移增加了 36%，并且与 SDF-1 α 产生的增加相关。 AMD3100 治疗导致白细胞迁移受到抑制。结论：基质祖细胞促进白细胞迁移，这似乎是通过 SDF-1 α 产生介导的。 SDF-1 α 的 SPC 产生可能受到伤口愈合过程中微环境中存在的其他细胞因子的调节。总之，这些观察结果提供了一种潜在机制，SPC 可以通过增强炎症细胞和组织祖细胞向组织损伤区域的募集来增强愈合。 (C) 2008。爱思唯尔公司。保留所有权利。
Background/Purpose: Stromal progenitor cells (SPC) enhance tissue repair in a variety of injury models. However, the mechanisms by which SPCs facilitate tissue repair remain poorly understood. We hypothesized that SPC-enhanced tissue repair is, in part, because of SPC-mediated recruitment of circulating cells to areas of tissue injury. To test this, we examined the migration of leukocytes in response to SPC in vitro.Methods: Leukocyte migration was assessed in response to SPC, SPC + transforming growth factor (TGF)-beta 1, or SPC + AMD3100 using a Transwell assay system (Coming, distributed by Fisher Scientific, Pittsburgh, PA). Supernatants were collected from lower chambers and analyzed for leukocyte content, leukocyte viability, and stromal-derived growth factor (SDF)-1 alpha concentration.Results: Stromal progenitor cells increased leukocyte migration compared to media alone (450 70 vs 112 +/- 17 cells/mu L; P < .05). SPC treatment with TGF-beta 1 resulted in a 36% increase in leukocyte migration and correlated with an increase in SDF-1 alpha production. Treatment with AMD3100 resulted in inhibition of leukocyte migration.Conclusions: Stromal progenitor cells promote leukocyte migration, and this appears to be mediated through SDF-1 alpha production. The SPC production of SDF-1 alpha may be modulated by other cytokines present in the microenvironment during wound healing. Together, these observations provide a potential mechanism by which SPC may augment healing through enhanced recruitment of inflammatory cells and tissue progenitor cells to areas of tissue injury. (C) 2008. Elsevier Inc. All rights reserved.