Complete resonance assignment of a natural abundance solid peptide by through-bond heteronuclear correlation solid-state NMR
Complete resonance assignment of a natural abundance solid peptide by through-bond heteronuclear correlation solid-state NMR
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DOI：
10.1021/ja0018320
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发表时间：
2000-10-11
影响因子：
15
通讯作者：
Emsley, L
中科院分区：
文献类型：
--
作者：
Lesage, A;Charmont, P;Emsley, L
天然丰度中等大小固态有机分子的核磁共振谱的归属仍然是一个具有挑战性的问题。在本文中，我们表明，所有的碳-13，氮-15，和质子NMR线的天然丰度固体肽的一个完整的分配可以通过结合各种单键和多键相关技术旋转固体。MAS光谱的分配是明确的，相对简单。
The assignment of the NMR spectra of natural abundance medium-sized solid-state organic molecules still represents a challenging problem. In this paper, we show that a complete assignment of all the carbon-13, nitrogen-15, and proton NMR lines of a natural abundance solid peptide can be performed by combining various one-bond and multiple-bond correlation techniques for rotating solids. The assignment of the MAS spectra is shown to be unambiguous and relatively straightforward.