Development of a radioimmunoassay for rat liver metallothionein-I and its application to the analysis of rat plasma and kidneys.
Development of a radioimmunoassay for rat liver metallothionein-I and its application to the analysis of rat plasma and kidneys.
复制标题
大鼠肝脏金属硫蛋白-I 放射免疫测定法的开发及其在大鼠血浆和肾脏分析中的应用。
DOI：
10.1042/bj2130459
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
I. Bremner
中科院分区：
文献类型：
--
作者：
R. Mehra;I. Bremner
用金属硫蛋白-兔免疫球蛋白G（IgG）偶联物免疫绵羊，获得高效价抗体，建立了测定大鼠肝金属硫蛋白-I的放射免疫分析方法。该测定对金属硫蛋白-I具有特异性，并且不依赖于与蛋白质结合的特定金属。与大鼠肝金属硫蛋白-II无明显交叉反应。使用的测定来测量金属硫蛋白在大鼠血浆和肾脏中的浓度进行了描述。
A sensitive radioimmunoassay for rat liver metallothionein-I has been developed using avid and high-titre antibodies obtained from sheep that were immunized with a conjugate of metallothionein and rabbit immunoglobulin G. The assay was specific for metallothionein-I, and did not depend on the particular metal bound to the protein. There was no significant cross-reaction with rat liver metallothionein-II. The use of the assay to measure metallothionein concentrations in rat plasma and kidneys is described.