Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms.
Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms.
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DOI：
10.1111/j.1742-4658.2009.06893.x
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发表时间：
2009-03
期刊：
影响因子：
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通讯作者：
Critchley DR
中科院分区：
文献类型：
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作者：
Debrand E;El Jai Y;Spence L;Bate N;Praekelt U;Pritchard CA;Monkley SJ;Critchley DR
Talins是连接粘附分子整合素家族与f -肌动蛋白的大型接头蛋白。在脊椎动物中，有两个talin基因。Talin 1对整合素介导的细胞粘附至关重要；talin 2的作用尚不清楚。在这里，我们报告了哺乳动物talin 2的详细分析。这揭示了一个与CpG岛相关的先前未被识别的启动子的存在，该启动子与第一个编码外显子被许多选择性拼接的非编码外显子分开，这些外显子的长度为100 ~ 200 kb。对小鼠基因诱捕系的分析表明，该启动子在成年组织中表达了大部分talin 2。我们还发现，睾丸和肾脏表达缺乏该蛋白n端一半的截断talin 2亚型，并为细长精子中短睾丸特异性talin 2亚型的发育调节表达提供了证据。最后，我们确定了talin 2编码区内的四个组织特异性剪接事件。
Talins are large adaptor proteins that link the integrin family of adhesion molecules to F-actin. In vertebrates, there are two talin genes. Talin 1 is essential for integrin-mediated cell adhesion; the role of talin 2 is unclear. Here we report a detailed analysis of mammalian talin 2. This reveals the existence of a previously unrecognized promoter associated with a CpG island, and separated from the first coding exon by numerous alternatively spliced noncoding exons spanning > 200 kb. Analysis of a mouse gene trap line shows that this promoter accounts for most of the talin 2 expression in adult tissues. We also demonstrate that testis and kidney express truncated talin 2 isoforms that lack the N-terminal half of the protein, and provide evidence for the developmentally regulated expression of the short testis-specific talin 2 isoform in elongating spermatids. Finally, we identify four tissue-specific alternative splicing events within the coding region of talin 2.