Identification of a nucleo-cytoplasmic ionic pathway by osmotic shock in isolated mouse liver nuclei
Identification of a nucleo-cytoplasmic ionic pathway by osmotic shock in isolated mouse liver nuclei
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通过渗透压休克鉴定离体小鼠肝核中的核-细胞质离子途径
DOI：
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发表时间：
2007
影响因子：
2.4
通讯作者：
M. Mazzanti
中科院分区：
文献类型：
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作者：
B. Innocenti;M. Mazzanti
核膜外膜含有离子通道的观察提出了一个问题，即这些电导是否在胞质和核膜池之间或胞质和细胞质之间进行通信。用膜片钳技术未能检测到大的、非选择性的孔，这导致了离子通道和核孔实际上是相同的推测。在本文中，我们提出的证据表明，离子通道，记录在离体肝细胞核的膜片钳构型的"核附着"，跨越双层膜的包膜，提供了一个直接接触之间的核质和细胞质。
SummaryThe observation that the nuclear envelope outer mem brane contains ion channels raises the question of whether these conductances communicate between the cytosol and the nuclear envelope cisternae or between the cytosol and the cytoplasm. Failure to detect large, nonselective holes using the patch-clamp technique has led to the speculation that ion channels and nuclear pores are in fact the same.In this paper we present evidence that the ionic channel, recorded in isolated liver nuclei with the patch-clamp configura tion of “nucleus-attached,” spans the double membrane of the envelope, providing a direct contact between nucleoplasm and cytoplasm.
胚胎鸡心室细胞自发心跳过程中的Na通道动力学。
影响因子：
3.4
作者：
Mazzanti,M;DeFelice,LJ
通讯作者：
DeFelice,LJ