Differential effects of cream, glucose, and orange juice on inflammation, endotoxin, and the expression of Toll-like receptor-4 and suppressor of cytokine signaling-3.
Differential effects of cream, glucose, and orange juice on inflammation, endotoxin, and the expression of Toll-like receptor-4 and suppressor of cytokine signaling-3.
复制标题
DOI：
10.2337/dc09-1630
复制
发表时间：
2010-05
期刊：
影响因子：
16.2
通讯作者：
Dandona P
中科院分区：
文献类型：
--
作者：
Deopurkar R;Ghanim H;Friedman J;Abuaysheh S;Sia CL;Mohanty P;Viswanathan P;Chaudhuri A;Dandona P
我们最近发现，除了氧化应激和细胞炎症外，高脂高碳水化合物（HFHC）膳食还诱导单核细胞（MNC）中内毒素（脂多糖[LPS]）血浆浓度和Toll样受体-4（TLR-4）和细胞因子信号转导抑制因子-3（SOCS 3）表达增加。饱和脂肪和碳水化合物，HFHC膳食的组分，已知诱导氧化应激和炎症，也诱导LPS，TLR-4和SOCS 3的增加。空腹的正常受试者被给予300卡路里的葡萄糖饮料，饱和脂肪如奶油，橙子汁，或只喝水。在0、1、3和5 h采集血样进行分析。炎症指标包括核因子-κB（NF-κB）结合、SOCS 3、肿瘤坏死因子-α（TNF-α）和白细胞介素（IL）-1β在摄入葡萄糖和奶油后显著增加，但TLR-4表达和血浆LPS浓度仅在摄入奶油后增加。橙子汁或水的摄入没有引起任何测量指标的变化。虽然葡萄糖和奶油都诱导NF-κB结合和SOCS 3、TNF-α和IL-1β在MNC中表达的增加，但只有奶油引起LPS浓度和TLR-4表达的增加。等量的橙子汁或水没有引起这些指标的变化。这些变化与动脉粥样硬化和胰岛素抵抗的发病机制有关。
We have recently shown that a high-fat high-carbohydrate (HFHC) meal induces an increase in plasma concentrations of endotoxin (lipopolysaccharide [LPS]) and the expression of Toll-like receptor-4 (TLR-4) and suppresser of cytokine signaling-3 (SOCS3) in mononuclear cells (MNCs) in addition to oxidative stress and cellular inflammation. Saturated fat and carbohydrates, components of the HFHC meal, known to induce oxidative stress and inflammation, also induce an increase in LPS, TLR-4, and SOCS3. Fasting normal subjects were given 300-calorie drinks of either glucose, saturated fat as cream, orange juice, or only water to ingest. Blood samples were obtained at 0, 1, 3, and 5 h for analysis. Indexes of inflammation including nuclear factor-κB (NF-κB) binding, and the expression of SOCS3, tumor necrosis factor-α (TNF-α), and interleukin (IL)-1β in MNCs, increased significantly after glucose and cream intake, but TLR-4 expression and plasma LPS concentrations increased only after cream intake. The intake of orange juice or water did not induce any change in any of the indexes measured. Although both glucose and cream induce NF-κB binding and an increase in the expression of SOCS3, TNF-α, and IL-1β in MNCs, only cream caused an increase in LPS concentration and TLR-4 expression. Equicaloric amounts of orange juice or water did not induce a change in any of these indexes. These changes are relevant to the pathogenesis of atherosclerosis and insulin resistance.