A family of genus-specific RNAs in tandem with DNA-binding proteins control expression of the badA major virulence factor gene in Bartonella henselae.
A family of genus-specific RNAs in tandem with DNA-binding proteins control expression of the badA major virulence factor gene in Bartonella henselae.
复制标题
一个属特异性 RNA 家族与 DNA 结合蛋白一起控制汉赛巴尔通体中 badA 主要毒力因子基因的表达。
DOI：
10.1002/mbo3.420
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发表时间：
2017
期刊：
影响因子：
3.4
通讯作者：
Anderson,Burt
中科院分区：
文献类型：
--
作者：
Tu,Nhan;Carroll,RonanK;Weiss,Andy;Shaw,LindseyN;Nicolas,Gael;Thomas,Sarah;Lima,Amorce;Okaro,Udoka;Anderson,Burt
亨selae巴尔通体是一种革兰氏阴性的人畜共患细菌，可引起人类感染，包括心内膜炎和细菌性血管瘤病。henselae已被证明在体外生长成大聚集体并形成生物膜。聚集生长和血管生成宿主反应需要三聚体自转运体粘附素BadA。我们检测了休斯顿- 1型乙型肝炎病毒株的转录组。利用RNA‐seq揭示了9个新的、高度表达的基因间转录物（巴尔通体调节转录物，Brt1‐9）。Brt家族的rna是巴尔通体属特有的，范围从194到203个核苷酸，具有高度同源性和稳定的预测二级结构。这9个RNA基因的下游是一个螺旋-螺旋-螺旋DNA结合蛋白（转录调节蛋白，Trp1 - 9），在用于RNA - seq的生长条件下转录不良。通过敲低或过表达菌株，我们发现Brt1和Trp1在bada调控和生物膜形成中都有作用。基于这些数据，我们假设Brt1是一种反式作用的sRNA，也作为反式作用的核糖开关来控制bada的表达。这个rna家族与下游的Trp DNA结合蛋白代表了一个新的协调调节回路，控制巴尔通体中毒力相关基因的表达。
Bartonella henselaeis a gram‐negative zoonotic bacterium that causes infections in humans including endocarditis and bacillary angiomatosis.B. henselaehas been shown to grow as large aggregates and form biofilms in vitro. The aggregative growth and the angiogenic host response requires the trimeric autotransporter adhesin BadA. We examined the transcriptome of the Houston‐1 strain ofB. henselaeusing RNA‐seq revealing nine novel, highly‐expressed intergenic transcripts (Bartonellaregulatory transcript, Brt1‐9). The Brt family of RNAs is unique to the genusBartonellaand ranges from 194 to 203 nucleotides with high homology and stable predicted secondary structures. Immediately downstream of each of the nine RNA genes is a helix‐turn‐helix DNA‐binding protein (transcriptional regulatory protein, Trp1‐9) that is poorly transcribed under the growth conditions used for RNA‐seq. Using knockdown or overexpressing strains, we show a role of both the Brt1 and Trp1 in the regulation ofbadAand also in biofilm formation. Based on these data, we hypothesize that Brt1 is atrans‐acting sRNA that also serves as acis‐acting riboswitch to control the expression ofbadA. This family of RNAs together with the downstream Trp DNA‐binding proteins represents a novel coordinated regulatory circuit controlling expression of virulence‐associated genes in the bartonellae.