Wearable Collector for Noninvasive Sampling of SARS-CoV-2 from Exhaled Breath for Rapid Detection
Wearable Collector for Noninvasive Sampling of SARS-CoV-2 from Exhaled Breath for Rapid Detection
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DOI：
10.1021/acsami.1c09309
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发表时间：
2021-08-24
影响因子：
9.5
通讯作者：
Demirci, Utkan
中科院分区：
文献类型：
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作者：
Soto, Fernando;Ozen, Mehmet Ozgun;Demirci, Utkan
呼出病毒的空气传播可以迅速传播，从而增加疾病从局部事件到大流行的进展。由于新冠肺炎大流行，各州和地方政府已强制在公共场所和工作场所使用防护口罩，以将疾病传播降至最低。在这里，我们利用了防护面罩的功能来进行新冠肺炎诊断。我们开发了一款用户友好、价格实惠、可穿戴的收集器。这种非侵入性的平台被集成到防护口罩中，以防止在佩戴期间呼气中收集空气传播的病毒。将病毒样本喷洒到收集器中以模拟空气传播，然后从收集器中提取浓缩的病原体用于进一步的分析评估。为了验证这一设计，定性比色环介导恒温扩增、定量逆转录聚合酶链式反应和基于抗体的斑点印迹分析用于检测SARS-CoV-2的存在。我们预计，这一平台将有助于对目前的SARS-CoV-2进行抽样，并有可能广泛适用于未来新出现的大流行的其他空气传播疾病。
Airborne transmission of exhaled virus can rapidly spread, thereby increasing disease progression from local incidents to pandemics. Due to the COVID-19 pandemic, states and local governments have enforced the use of protective masks in public and work areas to minimize the disease spread. Here, we have leveraged the function of protective face coverings toward COVID-19 diagnosis. We developed a user-friendly, affordable, and wearable collector. This noninvasive platform is integrated into protective masks toward collecting airborne virus in the exhaled breath over the wearing period. A viral sample was sprayed into the collector to model airborne dispersion, and then the enriched pathogen was extracted from the collector for further analytical evaluation. To validate this design, qualitative colorimetric loop-mediated isothermal amplification, quantitative reverse transcription polymerase chain reaction, and antibody-based dot blot assays were performed to detect the presence of SARS-CoV-2. We envision that this platform will facilitate sampling of current SARS-CoV-2 and is potentially broadly applicable to other airborne diseases for future emerging pandemics.