Pathogenicity of Frog Virus 3-like Virus in Red-eared Slider Turtles (Trachemys scripta elegans) at Two Environmental Temperatures
Pathogenicity of Frog Virus 3-like Virus in Red-eared Slider Turtles (Trachemys scripta elegans) at Two Environmental Temperatures
复制标题
DOI：
10.1016/j.jcpa.2013.01.007
复制
发表时间：
2013-08-01
影响因子：
0.8
通讯作者：
Driskell, E. A.
中科院分区：
文献类型：
--
作者：
Allender, M. C.;Mitchell, M. A.;Driskell, E. A.
蛙病毒疾病已影响多种爬行动物，但疾病进展和死亡率与环境温度的关系尚未确定。在这项研究中，两项单独的试验在 22 摄氏度 (n = 4) 和 28 摄氏度 (n = 4) 的环境温度下用蛙病毒（青蛙病毒 3 样病毒；FV3）分离物对成年雌性红耳龟（Trachemys scripta elegans）进行了挑战。 22℃和28℃试验中海龟的死亡率分别为100%和50%。在22℃下暴露于FV3的龟的中位生存时间为24天，而在28℃下接种的组中为30天。仅在22℃下接种的组中观察到一致的显微镜损伤，包括脾脏中血管的纤维蛋白样坏死、肝脏中的血管和窦状血栓、坏死性肌炎和轻度异嗜性。 间质性肺炎。定量聚合酶链反应针对主要衣壳蛋白的保守部分，能够检测全血、口腔和泄殖腔拭子、舌头、骨骼肌、肺、心脏、肝脏、脾脏、卵巢和肾脏中的病毒拷贝。死前临床样本中的病毒拷贝数在全血中不显着最高，而肾脏在死后样本中的病毒拷贝数最高。与 28 摄氏度下暴露于 FV3 的海龟相比，在 22 摄氏度下暴露于 FV3 的所有样本中的病毒拷贝数都较高。这项研究确定环境温度会影响感染蛙病毒的红耳龟的生存和疾病进展，这将有助于在临床或自由放养环境中管理动物。 (C) 2013 Elsevier Ltd. 保留所有权利。
Ranaviral disease has affected several species of reptiles, but disease progression and mortality in relation to environmental temperature has yet to be determined. In this study, two separate trials challenged adult female red-eared slider turtles (Trachemys scripta elegans) with a ranavirus (frog virus 3-like virus; FV3) isolate at environmental temperatures of 22 degrees C (n = 4) and 28 degrees C (n = 4). The mortality rates in the turtles in the 22 degrees C and 28 degrees C trials were 100% and 50%, respectively. Median survival time for turtles exposed to FV3 at 22 degrees C was 24 days, while it was 30 days in the group kept at 28 degrees C. Consistent microscopical lesions were observed only in the group inoculated at 22 degrees C and included fibrinoid necrosis of vessels in the spleen, vascular and sinusoidal thrombi in the liver, necrotizing myositis and a mild heterophilic interstitial pneumonia. Quantitative polymerase chain reaction, targeting a conserved portion of the major capsid protein, was able to detect virus copies in whole blood, oral and cloacal swabs, tongue, skeletal muscle, lung, heart, liver, spleen, ovary and kidney. Viral copy number in ante-mortem clinical samples was non-significantly highest in whole blood, while kidney had the highest viral copy number in post-mortem samples. All samples had higher virus copy number in turtles exposed to FV3 at 22 degrees C compared with 28 degrees C. This study determined that environmental temperature affects the survival and disease progression in ranavirus-infected red-eared slider turtles, which will aid in managing animals in a clinical or free-ranging setting. (C) 2013 Elsevier Ltd. All rights reserved.