Applications of Bioluminescence-Optogenetics in Rodent Models.
Applications of Bioluminescence-Optogenetics in Rodent Models.
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生物发光-光遗传学在啮齿动物模型中的应用。
DOI：
10.1007/978-1-0716-2473-9_27
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Berglund,Ken
中科院分区：
文献类型：
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作者：
Stern,MatthewA;Skelton,Henry;Fernandez,AlejandraM;Gutekunst,Claire-AnneN;Gross,RobertE;Berglund,Ken
在前一章中，我们介绍了生物发光-光遗传学（BL-OG）和发光蛋白融合蛋白（LMOs），这是一种新兴的分子神经调节方法。除了回顾BL-OG的基本原理外，我们还讨论了其体外应用，包括体外培养的细胞系和原代细胞。BL-OG是由一种易于扩散的分子介导的，当在啮齿动物中全身应用时，底物可以扩散到整个身体，包括大脑，即使在清醒和行为的动物中也可以方便地实现强大的分子神经调节。在本章中，我们提供了一个实用的指南BL-OG和LMO应用在啮齿动物模型的神经系统，无论是在体外和体内。
In the preceding chapter, we introduced bioluminescence-optogenetics (BL-OG) and luminopsin fusion proteins (LMOs), an emerging method of molecular neuromodulation. In addition to reviewing the fundamental principles of BL-OG, we provided a discussion of its application in vitro, including with cell lines and primary cells in culture in vitro. BL-OG is mediated by an easily diffusible molecule, luciferin, and when applied systemically in rodents, the substrate can spread throughout the body, including the brain, achieving powerful molecular neuromodulation with convenience even in awake and behaving animals. In this chapter, we provide a practical guide for BL-OG and LMO applications in rodent models of the nervous system, both ex vivo and in vivo.