Proteoglycan and collagen sensitive MRI evaluation of normal and degenerated articular cartilage
Proteoglycan and collagen sensitive MRI evaluation of normal and degenerated articular cartilage
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DOI：
10.1016/j.orthres.2003.09.008
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发表时间：
2004-05-01
影响因子：
2.8
通讯作者：
Nieminen, MT
中科院分区：
文献类型：
--
作者：
Nissi, MJ;Töyräs, J;Nieminen, MT
定量磁共振成像（MRI）技术已经发展到表征关节软骨的结构和组成。特别是Gd-DTPA（2-）增强的T-1成像对软骨蛋白聚糖含量敏感，而T松弛时间映射表明胶原网络的完整性和排列。然而，这些技术检测软骨早期骨关节病变的能力尚未得到证实。在这项研究中，使用上述技术在体外研究了正常和自发退化的牛髌骨软骨样本（n = 32）。作为参考，测定邻近组织的力学、组织学和生化特性，并采用软骨质量指数（CQI）评分系统对每个样本的结构和功能完整性进行评分。随着软骨退变的进展，Gd-DTPA（2-）存在时，表面T增加（r = 0.494, p < 0.05），表面T和体积T减少（r = -0.681, -0.688 (p < 0.05)），具有统计学意义。Gd-DTPA（2-）增强T-1成像是组织完整性的最佳预测指标，约占CQI变化的50%。目前的结果表明，定量MRI参数的变化表明了自发退行性关节软骨的结构和组成改变以及机械损伤。(C) 2004骨科研究学会。Elsevier Ltd.出版。版权所有。
Quantitative magnetic: resonance imaging (MRI) techniques have earlier been developed to characterize the structure and composition of articular cartilage. Particularly, Gd-DTPA(2-)-enhanced T-1 imaging is sensitive to cartilage proteoglyean content, while T relaxation time mapping is indicative of the integrity and arrangement of the collagen network. However, the ability of these techniques to detect: early osteoarthrotic changes in cartilage has not been demonstrated. In this study, normal and spontaneously degenerated bovine patellar cartilage samples (n = 32) were investigated in vitro using the aforementioned techniques. For reference, mechanical, histological and biochemical properties of the adjacent tissue were determined, and a grading system, the cartilage quality index (CQI), was used to score the structural and functional integrity of each sample. As cartilage degeneration progressed, a statistically significant increase in the superficial T (r = 0.494, p < 0.05) and a decrease in superficial and bulk T, in the presence of Gd-DTPA(2-) (r = -0.681 and -0.688 (p < 0.05), respectively) were observed. Gd-DTPA(2-)-enhanced T-1 imaging served as the best predictor of tissue integrity and accounted for about 50% of the variation in CQI. The present results reveal that changes in the quantitative MRI parameters studied are indicative of structural and compositional alterations as well as the mechanical impairment of spontaneously degenerated articular cartilage. (C) 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.