Cloning of the cDNA (DSC1) coding for human type 1 desmocollin and its assignment to chromosome 18.
Cloning of the cDNA (DSC1) coding for human type 1 desmocollin and its assignment to chromosome 18.
复制标题
克隆编码人 1 型桥粒胶蛋白的 cDNA (DSC1) 并将其分配至 18 号染色体。
作者：
I. King;J. Arnemann;N. Spurr;R. Buxton
桥粒是粘附性上皮连接，含有两种不同类别的钙粘蛋白相关糖蛋白（桥粒芯糖蛋白和桥粒胶蛋白），两者均以几种不同的亚型出现，其表达与上皮分化相关。我们现在已经分离出编码人桥粒胶蛋白的 cDNA 克隆，该蛋白在人表皮的分化程度更高的层中表达。该亚型与先前分离的人（3 型）桥粒胶蛋白具有 53% 的氨基酸同一性，后者在表皮基底层中表达。然而，成熟蛋白质的 N 端和 C 端更加高度保守。使用一组体细胞杂交体，人类 1 型桥粒糖蛋白（基因 DSC1）已被分配到 18 号染色体，与其他桥粒糖蛋白基因 (DSC3) 和已绘制的三个桥粒糖蛋白 (DSG) 基因位于同一位置。
Desmosomes are adhesive epithelial junctions that contain two distinct classes of cadherin-related glycoproteins (desmogleins and desmocollins), both of which occur as several different isoforms whose expression is related to epithelial differentiation. We have now isolated cDNA clones encoding a human desmocollin that is expressed in the more differentiated layers of human epidermis. This isoform has 53% amino acid identity with the previously isolated human (type 3) desmocollin, which is expressed in the basal layers of the epidermis. However, the N- and C-termini of the mature proteins are more highly conserved. Using a panel of somatic cell hybrids, human type 1 desmocollin (gene DSC1) has been assigned to chromosome 18, the same location as the other desmocollin gene (DSC3) and the three desmoglein (DSG) genes already mapped.