The effects of salinity on nitrification using halophilic nitrifiers in a Sequencing Batch Reactor treating hypersaline wastewater.
The effects of salinity on nitrification using halophilic nitrifiers in a Sequencing Batch Reactor treating hypersaline wastewater.
复制标题
序批式反应器处理高盐废水时盐度对嗜盐硝化菌硝化作用的影响
DOI：
10.1038/srep24825
复制
发表时间：
2016-04-25
影响因子：
4.6
通讯作者：
Peng YZ
中科院分区：
文献类型：
--
作者：
Cui YW;Zhang HY;Ding JR;Peng YZ
随着含盐废水的产生和使用逐年增加，避免富营养化等环境问题变得至关重要。先前的一项研究确定了启动从河口沉积物驯化的嗜盐污泥的方法，以去除盐度为 30 g/L 的废水中的氮。这项研究扩展了探索盐度对脱氮影响的工作。这项研究表明，混合嗜盐菌群可以去除盐度为 30-85 g/L 的废水中的氮。动力学分析表明，根据高盐度选择的嗜盐硝化菌具有低Ks、μmax和特定铵氧化速率的特点。这解释了高盐度操作阶段除铵效率下降的原因。盐度抑制氨氧化菌（AOB）活性以及优势AOB的数量，但对AOB优势种没有显着影响。嗜盐污泥中最主要的三个AOB谱系是滨亚硝化单胞菌、欧洲亚硝化单胞菌和运动亚硝化球菌。欧洲亚硝化球菌和运动亚硝化球菌主要受盐度影响，而亚硝酸盐积累和氨负荷对运动亚硝化球菌和欧洲亚硝化球菌的丰度起关键作用。该研究提供了有关嗜盐硝化细菌种群变化的见解。
With annual increases in the generation and use of saline wastewater, the need to avoid environmental problems such as eutrophication is critical. A previous study identified ways to start up a halophilic sludge domesticated from estuarine sediments to remove nitrogen from wastewater with a salinity of 30 g/L. This investigation expands that work to explore the impact of salinity on nitrogen removal. This study demonstrated that the mixed halophilic consortia removed nitrogen from wastewater with a salinity of 30–85 g/L. A kinetic analysis showed that halophilic nitrifiers selected based on hypersalinity were characterized by lowKs, μmaxand specific ammonium oxidization rates. This explains the decrease in ammonium removal efficiency in the high salinity operational phases. Salinity inhibited ammonia oxidizing bacteria (AOB) activity, as well as the number of dominant AOB, but did not significantly affect the AOB dominant species. Three most dominant AOB lineages in the halophilic sludge wereNitrosomonas marina, Nitrosomonas europaea,andNitrosococcus mobilis. Nitrosomonas europaeaandNitrosococcus mobiliswere mainly affected by salinity, while nitrite accumulation and ammonia loading played the key role in determining the abundance ofNitrosococcus mobilisandNitrosococcus europaea. The study contributes insights about shifts in halophilic nitrifying bacterial populations.