Protective Effects of Selenium and Vitamin E Combination on Experimental Colitis in Blood Plasma and Colon of Rats
Protective Effects of Selenium and Vitamin E Combination on Experimental Colitis in Blood Plasma and Colon of Rats
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DOI：
10.1007/s12011-009-8518-3
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发表时间：
2010-07-01
影响因子：
3.9
通讯作者：
Uzunkoy, Ali
中科院分区：
文献类型：
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作者：
Bitiren, Muharrem;Karakilcik, Ali Ziya;Uzunkoy, Ali
溃疡性结肠炎会增加氧化损伤，并伴有氧自由基的产生。硒（Se）和维生素E是两种天然抗氧化剂。本研究旨在探讨硒和维生素E联合应用对大鼠乙酸性结肠炎的保护作用。实验分为3组，即第1组（对照组）、第2组（实验性结肠炎组）、第3组（5%乙酸2 ml+维生素E 100 mg/kg体重+硒0.2mg/kg体重）。测定血浆和结肠组织过氧化氢酶（CAT）、脯氨酰二肽酶（PRS）、髓过氧化物酶（MPO）活性、总抗氧化能力（TAC）、总氧化状态（TOS）、氧化应激指数（OSI）、总巯基（T-SH）。AA处理组结肠的肉眼和显微损伤明显增加（分别为p < 0.01和p < 0.01），而硒和维生素E处理组结肠的肉眼和显微损伤明显减少（分别为p < 0.05和p < 0.01）。AA、Se和维生素E对血浆和结肠组织中CAT和PRS活性均有显著影响（p < 0.05和p < 0.01）。结肠组织MPO活性在AA处理后显著升高（p < 0.01），而在Se和维生素E处理后显著降低（p < 0.05）。AA能显著提高血浆中TOS和OSI的含量（p < 0.5）。AA使结肠TAC和T-SH降低（p < 0.05），Se和维生素E使结肠TAC和T-SH升高（p < 0.05）。基于这些结果，硒和维生素E可能在预防醋酸引起的炎症相关的氧化损伤中发挥重要作用。
Ulcerative colitis increases oxidative damage accompanied by production of free oxygen radicals. Selenium (Se) and vitamin E are two natural antioxidants. The present study was undertaken to investigate the possible protective role of Se and vitamin E combination in experimental colitis induced by acetic acid (AA) in rats. This study was carried out on three groups, namely the first (control), the second (experimental colitis group, 2 ml 5% acetic acid), and the third groups (2 ml 5% acetic acid, vitamin E (100 mg/kg body weight (bw)) plus Se (0.2 mg/kg bw)). The activities of catalase (CAT), prolidase (PRS), myeloperoxidase (MPO), total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), total thiol (T-SH) were determined in plasma and colon samples. Macroscopic and microscopic damages in colon were increased by AA treatment (p < 0.01 and p < 0.01, respectively), whereas they were decreased by selenium and vitamin E treatment (p < 0.05 and p < 0.01, respectively). The activities of CAT and PRS in the plasma and colon were significantly affected (p < 0.05 and p < 0.01) by treatment of AA, Se, and vitamin E. MPO activity in colon was increased (p < 0.01) by AA treatment and decreased (p < 0.05) by Se and vitamin E administration. The values of TOS and OSI in plasma were increased (p < 0.5) by AA. The TAC and T-SH in colon were decreased (p < 0.05) by AA and increased (p < 0.05) by Se and vitamin E. Based upon these results, Se and vitamin E may play an important role in preventive indication of the oxidative damage associated by acetic acid caused inflammation.