Juvenile hormone regulates the differential expression of putative juvenile hormone esterases via methoprene-tolerant in non-diapause-destined and diapause-destined adult female beetle
Juvenile hormone regulates the differential expression of putative juvenile hormone esterases via methoprene-tolerant in non-diapause-destined and diapause-destined adult female beetle
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保幼激素通过甲虫橡胶耐受性在非滞育和滞育成年雌性甲虫中调节假定的保幼激素酯酶的差异表达
DOI：
10.1016/j.gene.2017.06.061
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发表时间：
2017
期刊：
影响因子：
3.5
通讯作者：
Wang Xiao-Ping
中科院分区：
文献类型：
--
作者：
Zhu Li;Yin Tian-Yan;Sun Dan;Liu Wen;Zhu Fen;Lei Chao-Liang;Wang Xiao-Ping
保幼激素 (JH) 在调节许多昆虫和甲壳类动物的蜕皮、变态、繁殖和滞育（休眠）方面发挥着重要作用。 JH酯酶（JHE）可以通过调节JH降解来控制JH滴度。尽管 JHE 的生物化学和结构已得到充分研究，但其表达调控仍不清楚。我们在卷心菜甲虫Colaphellus Bowringi中鉴定了三个假定的JHE（JHE1、JHE2、JHE3），并研究了JH信号在非滞育目的地（NDD，生殖）和滞育目的地（DD）雌性成虫中的表达调节。序列和系统发育树分析表明，这三种假定的 JHE 与其他昆虫和一种甲壳类动物的 JHE 共享保守基序，并且与鞘翅目、双翅目、直翅目、膜翅目和十足目 JHE 相似。然而，它们与半翅目和鳞翅目 JHE 的关系不太密切。JHE 在 NDD 雌性成虫中的表达高于 DD 雌性成虫。 JH类似物诱导DD雌性成虫显着上调JHE1和JHE2的表达，但对JHE3的表达没有影响。 NDD 雌性成虫中 JH 候选受体耐甲氧乙烯 (Met) 的敲除会下调所有三个 JHE 的表达。这些结果表明JHE表达与JH信号传导呈正相关，并且Met可能参与了JH介导的DD和NDD成年雌性C中JHE的差异表达。鲍林吉。
Juvenile hormone (JH) plays an essential role in regulating molting, metamorphosis, reproduction, and diapause (dormancy), in many insects and crustaceans. JH esterases (JHEs) can control JH titer by regulating JH degradation. Although the biochemistry and structure of JHEs have been well studied, regulation of their expression remains unclear. We identified three putativeJHEs(JHE1,JHE2,JHE3) in the cabbage beetleColaphellus bowringi, and investigated the regulation of their expression by JH signaling in non-diapause-destined (NDD, reproductive) and diapause-destined (DD) female adults. Sequence and phylogenetic tree analyses indicate that the three putative JHEs shared conserved motifs with the JHEs of other insects and one crustacean, and were similar to Coleopteran, Dipteran, Orthopteran, Hymenopteran, and Decapodan JHEs. They were, however, less closely related to Hemipteran and Lepidopteran JHEs.JHEswere more highly expressed in NDD female adults than in DD female adults. JH analog induction in DD female adults significantly upregulated the expression ofJHE1andJHE2, but had no effect on the expression ofJHE3. Knockdown of the JH candidate receptormethoprene-tolerant(Met) in NDD female adults downregulated the expression of all threeJHEs. These results suggest thatJHEexpression is positively correlated with JH signaling, and thatMetmay be involved in the JH-mediated differential expression ofJHEin DD and NDD adult femaleC. bowringi.