Octavolateral projections and organization in the medulla of a teleost fish, the sleeper goby (Dormitator latifrons)
Octavolateral projections and organization in the medulla of a teleost fish, the sleeper goby (Dormitator latifrons)
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DOI：
10.1002/cne.20363
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发表时间：
2005-01-03
影响因子：
2.5
通讯作者：
Lu, ZM
中科院分区：
文献类型：
--
作者：
Tomchik, SM;Lu, ZM
这项研究是第一次采用同时标记不同颜色的荧光染料和共聚焦显微镜，以调查在任何鱼类的octavolateral神经的中央投射。三维重建的后脑octavolateral核和重叠的octavolateral的预测进行了评估，在硬骨鱼，睡眠虾虎鱼（Dormitator latifrons）。支配耳石器、半规管和侧线的八侧神经以多种复杂的模式投射到七个后脑核团。延髓通常由前庭区背侧的听觉区组成。下降的octaval核（DON）的中间分部接收来自耳石器官的交错投射，背内侧DON可能整合了多个听觉输入。传入的三个耳石器官（椭圆囊，球囊，和lagena）项目的中间DON在大约相等的比例，支持生理证据表明，所有三个耳石器官的睡眠虾虎鱼的听觉角色。前八极核接受来自八极体外侧器官的部分分离输入。背侧分裂的大细胞octaval核（MgON）接收高度重叠的耳石器和半规管输入，我们建议，这一地区是一个主要的octaval整合中心。延髓腹侧区域（切向八值核、腹侧DON和腹侧MgON）主要接受椭圆囊和半规管输入，表明前庭的作用。每个半规管神经都投射到后脑的不同区域，在大多数八值核中几乎没有重叠。传出神经元接受双侧输入并单侧投射到八侧器官。(C)2004 Wiley-Liss，Inc.
This study is the first to employ simultaneous labeling with different colored fluorescent dyes and confocal microscopy to investigate the central projections of the octavolateral nerves in any fish. Three-dimensional reconstructions of the hindbrain octavolateral nuclei were made and overlap of octavolateral projections was assessed in a teleost, the sleeper goby (Dormitator latifrons). The octavolateral nerves, which innervate the otolithic organs, semicircular canals, and lateral lines, project to seven hindbrain nuclei in diverse, complex patterns. The medulla is generally organized with auditory regions dorsal to vestibular regions. The intermediate subdivision of the descending octaval nucleus (DON) receives interdigitating projections from the otolithic organs, and the dorsomedial DON likely integrates multiple auditory inputs. Afferents from the three otolithic organs (the utricle, saccule, and lagena) project to the intermediate DON in approximately equal proportion, supporting physiological evidence that suggests auditory roles for all three otolithic organs in the sleeper goby. The anterior octaval nucleus receives partially segregated inputs from the octavolateral organs. The dorsal division of the magnocellular octaval nucleus (MgON) receives highly overlapping otolithic organ and semicircular canal input, and we propose that this region is a major octaval integration center. Regions in the ventral medulla (the tangential octaval nucleus, ventral DON, and ventral MgON) receive mainly utricular and semicircular canal inputs, suggesting vestibular roles. Each semicircular canal nerve projects to distinct regions of the hindbrain, with little overlap in most octaval nuclei. Efferent neurons receive bilateral input and project unilaterally to the octavolateral organs. (C) 2004 Wiley-Liss, Inc.