Alpaca semen quality in relation to different diets
Alpaca semen quality in relation to different diets
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DOI：
10.1071/rd12050
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发表时间：
2013-01-01
影响因子：
1.9
通讯作者：
Stelletta, C.
中科院分区：
文献类型：
--
作者：
Juyena, N. S.;Vencato, J.;Stelletta, C.
本研究旨在评价饲喂不同饲粮（干草、干草+牧草、牧草+羊精、牧草+马精、周期分别为1-4）的羊驼精浆生化组成及精液质量。羊驼（n = 5）饲喂4种不同的饲粮，每种饲粮饲喂6周。在饲喂饲粮期间，采用人工阴道采集精液，测定其体积、粘度、精子浓度和精子活力。评估睾丸体积和体况评分。对精浆进行生化分析，测定总蛋白、甘油三酯、胆固醇、γ -谷氨酰转移酶、丙氨酸转氨酶（ALT）和碱性磷酸酶水平。用一维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳研究了蛋白质谱。在相同饮食的不同雄性之间，精液参数有很大的差异。饲粮中添加商品羊、马精料显著提高了羊驼的精液量（P < 0.05）。第1 ~ 4期精子浓度和活动力显著降低（P < 0.05）。饮食变化对黏度没有影响。从第1期到第4期，甘油三酯和胆固醇含量以及γ -谷氨酰转移酶、ALT和碱性磷酸酶浓度显著降低。无论实验时间如何，羊驼之间的精浆酶浓度变化很大，而饮食对精浆中葡萄糖和总蛋白浓度没有影响。经图像分析，电泳凝胶中有8条分子量为200 ~ 14 kDa的蛋白条带。所有羊驼精浆样品中均不存在14-kDa（总蛋白在md - dL（-1）中表达，分子量为14-kDa， TP8）和21-kDa（总蛋白在md - dL（-1）中表达，分子量为21-kDa， TP7）条带的蛋白质片段。在4个周期内，任何蛋白质组分的浓度都没有显著变化。此外，60-kDa（分子量为60-kDa的md - dL（-1）中表达的总蛋白，TP3）带的蛋白部分在所有时期都是最普遍的。这些结果表明，羊驼的精液质量以及与精浆成分有关的一些参数的变化与饲粮有关，这可能表明需要特定的饲粮配方来提高繁殖性能。我们假设，在羊驼中，一些生殖特征对喂养方案的反应变化的潜在机制可能与内分泌性腺系统的变化有关。
The aim of the present study was to evaluate the biochemical composition of seminal plasma, along with semen quality, of alpacas maintained on different diets (hay; hay + pasture grazing; pasture grazing + sheep concentrate; pasture grazing + horse concentrate; Periods 1-4, respectively). Alpacas (n = 5) were fed the four different diets for a period of 6 weeks each. During the period of feeding of each diet, semen was collected using an artificial vagina to determine its volume, viscosity, sperm concentration and sperm motility. Moreover, testicular volume and body condition score were evaluated. Seminal plasma was analysed biochemically to measure total protein, triglyceride, cholesterol, gamma-glutamyl transferase, alanine aminotransferase (ALT) and alkaline phosphatase levels. Protein profiles were investigated using one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. There was high variability in semen parameters between different males maintained on the same diet. Semen volume increased significantly (P < 0.05) when alpacas were fed diets containing commercial sheep and horse concentrates. In contrast, sperm concentration and motility decreased significantly (P < 0.05) from Period 1 to Period 4. Dietary changes had no effect on viscosity. Significant reductions were seen in triglyceride and cholesterol content, as well as gamma-glutamyl transferase, ALT and alkaline phosphatase concentrations, from Period 1 to Period 4. Regardless of experimental period, a wide variation was seen in seminal plasma enzyme concentrations between alpacas, whereas diet had no effect on glucose and total protein concentrations in the seminal plasma. Eight protein bands, with molecular weights ranging from 200 to 14 kDa, were considered in electrophoresis gel after image analysis. Proteins fractions of the 14-kDa (total protein express in md dL(-1) with a molecular weight of 14-kDa, TP8) and 21-kDa (total protein express in md dL(-1) with a molecular weight of 21-kDa, TP7) bands were not present in all samples of alpaca seminal plasma. There were no significant changes in the concentration of any protein fractions during the four periods. Moreover, the protein fraction of the 60-kDa (total protein express in md dL(-1) with a molecular weight of 60-kDa, TP3) band was the most prevalent in all periods. These results demonstrate that there are marked changes in semen quality, as well as some parameters related to the composition of alpaca seminal plasma, that are dependent on diet, which may indicate the need for specific diet formulation to improve reproductive performance. We hypothesise that, in alpacas, the mechanisms underlying the changes in some reproductive traits in response to feeding regimens could be related to changes in the endocrine-gonadal system.